LOCUS       sequence-116377-        1378 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zebrafish expression of a photoactivatable fluorescent calcium 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, 
            Rockwell NC, Martin SS, Lagarias JC, Isacoff EY
  TITLE     Photoactivatable genetically encoded calcium indicators for targeted
            neuronal imaging.
  JOURNAL   Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480.
  PUBMED    26167640
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480."
COMMENT     Sequence Label: DF-UAS-spa-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..1378
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(16..24,25..42,43..45,46..78,79..81,82..105,106..132,
                     133..189,190..195,196..465,466..483,484..918,919..924,
                     925..1368)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGP
                     VLLPDNHYLSHQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTFS
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      112..121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     402..423
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(529..550)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tataggcgcg ccaccatggg ttctcatcat catcatcatc atggtatggc tagcatgact
       61 ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatct cgccaccatg
      121 gtcgactcat cacgtcgtaa gtggaataag acaggtcacg cagtcagagc tataggtcgg
      181 ctgagctcac tcgagaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcg
      241 aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc agctcgccga ccactaccag
      301 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagccat
      361 cagtccgtgc tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      421 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg
      481 agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      541 gacggcgacg taaacggcca caagttcagc gtgaggggcg agggtgaggg cgatgccacc
      601 aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      661 accctcgtga ccaccttcag ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
      721 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      781 tccttcaagg acgacggcac ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      841 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      901 cacaagctgg agtacaacct gccggaccaa ctgactgaag agcagatcgc agaatttaaa
      961 gaggaattct ccctatttga caaggacggg gatgggacaa taacaaccaa ggagctgggg
     1021 acggtgatgc ggtctctggg gcagaacccc acagaagcag agctgcagga catgatcaat
     1081 gaagtagatg ccgacggtga cggcacaatc gacttccctg agttcctgac aatgatggca
     1141 agaaaaatga aatacaggga cacggaagaa gaaattagag aagcgttcgg tgtgtttgat
     1201 aaggatggca atggctacat cagtgcagca gagcttcgcc acgtgatgac aaaccttgga
     1261 gagaagttaa cagatgaaga ggttgatgaa atgatcaggg aagcagacat cgatggggat
     1321 ggtcaggtaa actacgaaga gtttgtacaa atgatgacag cgaagtgaac gcgttata
//
