LOCUS       sequence-116402-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses GFP-tagged RNAseH1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  TITLE     RNAseH1 plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFRT-TODestGFP_RNAseH1
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    30..48
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     58..82
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(179..181,182..184,185..697)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(224..245)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(485..504)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     832..853
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcagagctct cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc
       61 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      121 aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccat
      181 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      241 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      301 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      361 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
      421 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      481 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      541 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      601 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
      661 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
      721 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      781 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
      841 gctggagttc gtgaccgccg cccgggatca ctctcggcat ggacgagct
//
