LOCUS       sequence-116451-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET biosensor Destination vector for E. coli expression. Encodes a 
            FRET pair for fusion with ligand binding domain of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, 
            Frommer WB
  TITLE     Abscisic acid dynamics in roots detected with genetically encoded 
            FRET sensors.
  JOURNAL   eLife. 2014 Apr 15;3:e01741. doi: 10.7554/eLife.01741.
  PUBMED    24737862
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-04-15"; volume: "3:e01741. doi"; pages: " 
            10.7554/eLife.01741"
COMMENT     Sequence Label: pFLIP42
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..46
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             50..82
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(86..94,95..109)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(125..127,128..640)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(167..188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(428..447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agaaggagat atacatatgc ggggttctca tcatcatcat catcatggta tggctagcat
       61 gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccgggcct
      121 cgaggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      181 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      241 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      301 cctcgtgacc accttcggct acggcctgat gtgcttcgcc cgctaccccg accacatgaa
      361 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      421 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      481 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      541 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
      601 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      661 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      721 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      781 cc
//
