LOCUS       sequence-116459-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 D10A+D839A+H840A+N863A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675."
COMMENT     Sequence Label: Cas9m4
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
       61 accgggaccg atccagcctc cggactctag aggatcgaac ccttgccacc atggacaaga
      121 agtactccat tgggctcgct atcggcacaa acagcgtcgg ctgggccgtc attacggacg
      181 agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc cacagcataa
      241 agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa gccacgcggc
      301 tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc tacctgcagg
      361 agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg ctggaggagt
      421 cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc aatatcgtgg
      481 acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag aagcttgtag
      541 acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat atgatcaaat
      601 ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat gtcgacaaac
      661 tctttatcca actggttcag acttacaatc agcttttcga agagaacccg atcaacgcat
      721 ccggagttga c
//
