LOCUS       sequence-116475-         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of SUMO2(Q87R) with a strep and an HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Bursomanno S, McGouran JF, Kessler BM, Hickson ID, Liu Y
  TITLE     Regulation of SUMO2 target proteins by the proteasome in human cells
            exposed to replication stress.
  JOURNAL   J Proteome Res. 2015 Apr 3;14(4):1687-99. doi: 10.1021/pr500997p. 
            Epub 2015 Mar 19.
  PUBMED    25748227
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr500997p";
            journalName: "J Proteome Res"; date: "2015-04-3- 3"; volume: "14"; 
            issue: "4"; pages: "1687-99"
COMMENT     Sequence Label: pcDNA3.1-StrepHA-SUMO2(Q87R)
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..302)
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 2"
                     /label=SUMO2
                     /translation="MADEKPKEGVKTENNNHINLKVAGQDGSVVQFKIKRHTPLSKLMK
                     AYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFRQQTGG"
     CDS             complement(318..344)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(372..395)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(432..455)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(505..524)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(506..524)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(569..620)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(598..618)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 actcgagcgg ccgctcagta gacacctccc gtctgctgtc ggaacacatc aattgtatct
       61 tcatcctcca tttccaactg tgcaggtgtg tctgtttcat tgattggttg cccgtcaaat
      121 cggaatctga tctgcctcat tgacaatccc tgtcgttcac aataggcttt cattagttta
      181 ctaagtggtg tatgcctctt aatcttaaac tgcaccacag aaccatcctg ccccgccacc
      241 ttcaaattaa tatgattgtt gttctcagtc ttgactcctt ccttgggctt ttcgtcggcc
      301 atgatatccg ctgcagcggc gtaatcgggg acgtcataag ggtaagaatt cgggaccgcg
      361 gtctcggcgc ctttttcgaa ctgcgggtgg ctccacgatc cacctcccga tccacctccg
      421 gaacctccac ctttctcaaa ctgcgggtgg ctccagctag ccatggtacc aagcttaagt
      481 ttaaacgcta gccagcttgg gtctccctat agtgagtcgt attaatttcg ataagccagt
      541 aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc caccgtacac
      601 gcctaccgcc catttgcgtc aatggggcgg agttgttacg aca
//
