LOCUS       sequence-116510-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  two TALEN monomers in one plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Mariano A, Xu L, Han R
  TITLE     Highly efficient genome editing via 2A-coupled co-expression of two 
            TALEN monomers.
  JOURNAL   BMC Res Notes. 2014 Sep 10;7:628. doi: 10.1186/1756-0500-7-628.
  PUBMED    25204665
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2014-09-10"; volume: "7:628. doi"; pages: " 
            10.1186/1756-0500-7-628"
COMMENT     Sequence Label: PTAL10
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..86
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             96..161
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             168..188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagcaat gtactaacta cgctttgttg aaactcgctg
       61 gcgatgttga aagtaacccc ggtcctgaat tcatggacta caaagaccat gacggtgatt
      121 ataaagatca tgacatcgat tacaaggatg acgatgacaa gatggccccc aagaagaaga
      181 ggaaggtggg cattcacggg gtacctatgg tggatctacg cacgctcggc tacagtcagc
      241 agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac cacgaggcac
      301 tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac ccggcagcgt
      361 tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag gcgacacacg
      421 aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag gccttgctca
      481 cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa cttgtgaaga
      541 ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc aatgcactga
      601 cgggtgctcc cctggagacg tcgaccgtct ccaacgacca cctcgtcgcc ttggcctgcc
      661 tcggcggacg tcctgccatg gatgcagtga aaaagggatt gccgcacgcg ccggaattga
      721 tcagaagagt caatcgccgt attggcgaac gcacgtccca tcgcgttgcc ggatcccagc
      781 tggtcaaaag tgaactggag gagaagaaat ctgaacttcg tcataaattg aaatatgtgc
      841 ctcatgaata tattgaatta attgaaattg ccagaaatcc cactcaggat aggattcttg
      901 aaatgaaggt aatggaattt tttatgaaag tttatggata tagaggtgaa catttgggtg
      961 gatcaaggaa accggacgga gcaatttata ctgtcggatc tcctattgat tacggtgtga
     1021 tcgtggat
//
