LOCUS       sequence-116516-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  two TALEN monomers in one plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Mariano A, Xu L, Han R
  TITLE     Highly efficient genome editing via 2A-coupled co-expression of two 
            TALEN monomers.
  JOURNAL   BMC Res Notes. 2014 Sep 10;7:628. doi: 10.1186/1756-0500-7-628.
  PUBMED    25204665
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2014-09-10"; volume: "7:628. doi"; pages: " 
            10.1186/1756-0500-7-628"
COMMENT     Sequence Label: PTAL10
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..36
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(212..799)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNPTQDRILEMKV
                     MEFFMKVYGYRGEHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNRKTNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
       61 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
      121 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca
      181 gttatctaga tccggtggat cactcgagtt aaaagtttat ctcgccgtta ttaaatttcc
      241 ttctcacttc ctctaaggtt aatgtgccgg ctttaatcat ttctccacca attaaaagct
      301 cttctacact aagaacagct ccattacaat tagtcttacg atttaatcgt gtaagctgag
      361 ctttgtagtt tcctttaaag tgaccactca caaataaaaa cttaaattcc gttacagaag
      421 atggatagac tttccaccat tcattagggt tgatatgttt gtttcgtgtt tgattttcct
      481 tgacatatcg ttgcatttca tctgcttggc caattggcag attataacct ccgctataag
      541 ctttagtatc cacgatcaca ccgtaatcaa taggagatcc gacagtataa attgctccgt
      601 ccggtttcct tgatccaccc aaatgttcac ctctatatcc ataaactttc ataaaaaatt
      661 ccattacctt catttcaaga atcctatcct gagtgggatt tctggcaatt tcaattaatt
      721 caatatattc atgaggcaca tatttcaatt tatgacgaag ttcagatttc ttctcctcca
      781 gttcactttt gaccagctgg gatccggcaa cgcgatggga cgtgcgttcg ccaatacggc
      841 gattgactct tctgatcaat tccggcgcgt gcggcaatcc ctttttcact gcatccatgg
      901 caggacgtcc gccgaggcag gccaaggcga cgaggtggtc gttggagacc gtcgacggtc
      961 tccaggggag cacccgtcag tgcattgcgc gatgcatgca ctgcctccat tgcggtcacg
     1021 ccgccacgtt ttgcaatctt cacaagttgg cctg
//
