LOCUS       sequence-116665-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ManII reporter only (no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: ManII-SBP-mCherry_puromycin
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(348..367)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     617..635
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agcacactgg atcagttatc tatgcggccg cggatcctta attaattggc cctcgaggcc
       61 ttaaggccgg cccttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggcgcg
      121 ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt
      181 gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc
      241 agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc ccttcagggc
      301 gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat
      361 tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc
      421 gtcctgtagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac
      481 gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga tgtcggcggg
      541 gtgcttcacg taggccttgg agccgtacat gaactgaggg gacaggatgt cccaggcgaa
      601 gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
      661 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac
      721 cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcg
//
