LOCUS       sequence-116685-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of near-infrared PI(4,5)P2 biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Idevall-Hagren O, Dickson EJ, Hille B, Toomre DK, De Camilli P
  TITLE     Optogenetic control of phosphoinositide metabolism.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 
            10.1073/pnas.1211305109. Epub 2012 Jul 30.
  PUBMED    22847441
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1211305109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-08-28- 28"; volume: "109"; issue: "35"; pages: 
            "E2316-23"
COMMENT     Sequence Label: iRFP-PH-PLCdelta1
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..968
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
ORIGIN
        1 ccgtcagatc cgctagcacc atggctgaag gatccgtcgc caggcagcct gacctcttga
       61 cctgcgacga tgagccgatc catatccccg gtgccatcca accgcatgga ctgctgctcg
      121 ccctcgccgc cgacatgacg atcgttgccg gcagcgacaa ccttcccgaa ctcaccggac
      181 tggcgatcgg cgccctgatc ggccgctctg cggccgatgt cttcgactcg gagacgcaca
      241 accgtctgac gatcgccttg gccgagcccg gggcggccgt cggagcaccg atcactgtcg
      301 gcttcacgat gcgaaaggac gcaggcttca tcggctcctg gcatcgccat gatcagctca
      361 tcttcctcga gctcgagcct ccccagcggg acgtcgccga gccgcaggcg ttcttccgcc
      421 gcaccaacag cgccatccgc cgcctgcagg ccgccgaaac cttggaaagc gcctgcgccg
      481 ccgcggcgca agaggtgcgg aagattaccg gcttcgatcg ggtgatgatc tatcgcttcg
      541 cctccgactt cagcggcgaa gtgatcgcag aggatcggtg cgccgaggtc gagtcaaaac
      601 taggcctgca ctatcctgcc tcaaccgtgc cggcgcaggc ccgtcggctc tataccatca
      661 acccggtacg gatcattccc gatatcaatt atcggccggt gccggtcacc ccagacctca
      721 atccggtcac cgggcggccg attgatctta gcttcgccat cctgcgcagc gtctcgcccg
      781 tccatctgga attcatgcgc aacataggca tgcacggcac gatgtcgatc tcgattttgc
      841 gcggcgagcg actgtgggga ttgatcgttt gccatcaccg aacgccgtac tacgtcgatc
      901 tcgatggccg ccaagcctgc gagctagtcg cccaggttct ggcctggcag atcggcgtga
      961 tggaagagtg tacagagatc tcgagctcaa gcttcacggg ctccaggatg acccggacct
     1021 tcaggccctt ctgaagggca gccagcttct gaaggtgaag tccagctcgt ggcgtaggga
     1081 acgcttctac aagct
//
