LOCUS       sequence-116799-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Sec61-C-18
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..382
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="PGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGR
                     TTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     polyA_signal    543..664
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(580..599)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     634..653
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(808..829)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg gatctggctc
       61 cagcgcaggc agcgcatccg gcggaagcgg aagccctggt ccgaccccca gtggcactaa
      121 cgtgggatcc tcagggcgct ctcccagcaa agcagtggcc gcccgggcgg cgggatccac
      181 tgtccggcag aggaaaaatg ccagctgtgg gacaaggagt gcaggccgca caacctcggc
      241 aggcaccggg gggatgtggc gattctacac agaagattca cctgggctca aagttggccc
      301 tgttccagta ttggttatga gtcttctgtt catcgcttct gtatttatgt tgcacatttg
      361 gggcaagtac actcgttcgt aggaattctg cagtcgacgg taccgcgggc ccgggatcca
      421 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      481 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      541 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
      601 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      661 cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      721 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      781 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
//
