LOCUS       sequence-116865-        1208 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Outer Mitochondrial Membrane, Excitation: 590, 
            Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1208)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1208
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             491..1171
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     892..911
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1086..1109
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 taccgtcaga tccgctagcg ccaccatggt gggtcggaac agcgccatcg ccgccggtgt
       61 atgcggggcc cttttcattg ggtactgcat ctacttcgac cgcaaaagac gaagtgaccc
      121 caacttcaag aacaggcttc gagaacgaag aaagaaacag aagcttgcca aggagagagc
      181 tgggctttcc aagttacctg accttaaaga tgctgaagct gttcagaagt tcttccttga
      241 agaaatacag cttggtgaag agttactagc tcaaggtgaa tatgagaagg gcgtagacca
      301 tctgacaaat gcaattgctg tgtgtggaca gccacagcag ttactgcagg tcttacagca
      361 aactcttcca ccaccagtgt tccagatgct tctgactaag ctcccaacaa ttagtcagag
      421 aattgtaagt gctcagagct tggctgaaga tgatgtggaa ggcggtagcg gggatccacc
      481 ggtggctacc atggtgagca agggcgagga ggtcatcaag gagttcatgc gcttcaagga
      541 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
      601 cccctacgag ggcacccaga ccgccaggct gaaggtgacc aagggtggcc ccctgccctt
      661 cgcctgggac atcctgtccc ctcagatcat gtacggctcc aaggcctacg tgaagcaccc
      721 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
      781 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
      841 cgagttcatc tacaaggtga aggtgcgcgg caccaacttc ccctccgacg gccccgtaat
      901 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
      961 cctgaagggc gagatgaaga tgaggctgag gctgaaggac ggcggccact acgacgccga
     1021 ggtcaagacc acctacatgg ccaagaagcc cgtgcagctg cccggcgcct acaagaccga
     1081 catcaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
     1141 cgccgagggc cgccactcca ccggcgccta agcggccgcg actctagatc ataatcagcc
     1201 ataccaca
//
