LOCUS       sequence-116930-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Carries CA repeats of 50-100 nucleotides in length, an RBPMS target.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  TITLE     RNA-binding protein 3' UTR target plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDONR221_50_100_CA_repeat
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(256..272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(256..272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(269..291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    280..296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(304..310,311..328,329..334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    349..370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(487..504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(738..757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctagatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt caggccgtgt
       61 gtgtgtgtgt gtgtggtgcc gtgtgtgtgt gtgtgtgtgg tgccgtgtgt gtgtgtgtgt
      121 gtggtgccgt gtgtgtgtgt gtgcctgaat tccagcacac tggcggccgt tactagtgga
      181 tccgagctcg gtaccaagct tgatgcatag cttgagtatt ctaacgcgtc acctaaatag
      241 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      301 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      361 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      421 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      481 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      541 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      601 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgct
//
