LOCUS       sequence-116947-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase assay vector, assessing regulation of 50-100 nucleotide 
            CA repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  TITLE     RNA-binding protein 3' UTR target plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psicheck_50_100_CA_repeat
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          136..268
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     251..270
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        313..331
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      335..344
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
       61 tgcaaaaagc ttgattcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg
      121 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa
      181 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact
      241 gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa
      301 ggctagagta cttaatacga ctcactatag gctagccacc atggcttcca aggtgtacga
      361 ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg tgggctcgct gcaagcaaat
      421 gaacgtgctg gactccttca tcaactacta tgattccgag aagcacgccg agaacgccgt
      481 gatttttctg catggtaacg ctgcctccag ctacctgtgg aggcacgtcg tgcctcacat
      541 cgagcccgtg gctagatgca tcatccctga tctgatcgga atgggtaagt ccggcaagag
      601 cgggaatggc tcatatcgcc tcctggatca ctacaagtac ctcaccgctt ggttcgagct
      661 gctgaacctt ccaaagaaaa tcatctttgt gggccacgac tggggggctt gtctggcctt
      721 tcactactcc tacgagcacc
//
