LOCUS       sequence-117120-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses lambdaN-HA-tagged EDC4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Tritschler F, Braun JE, Motz C, Igreja C, Haas G, Truffault V, 
            Izaurralde E, Weichenrieder O
  TITLE     DCP1 forms asymmetric trimers to assemble into active mRNA decapping
            complexes in metazoa.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Dec 22. 106(51):21591-6.
  PUBMED    19966221
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Dec 22. 106(51):21591-6."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsEDC4
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          117..249
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     232..251
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        294..312
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      316..325
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             322..387
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             412..438
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
       61 ctgcagtgac tctcttaagg tagccttgca gaagttggtc gtgaggcact gggcaggtaa
      121 gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga
      181 gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc
      241 tctccacagg tgtccactcc cagttcaatt acagctctta aggctagagt acttaatacg
      301 actcactata ggctagccac catggacgca caaacacgac gacgtgagcg tcgcgctgag
      361 aaacaagctc aatggaaagc tgcaaaccca ccgctcgaga attcaatggc ttacccatac
      421 gatgttccag attacgctat ggcctcctgc gcgagcatcg acatcgagga cgccacgcag
      481 cacctgcggg acatcctcaa gctggaccgg cccgcgggcg gccccagtgc agagagccca
      541 cggccatcca gtgcctacaa tggggacctc aatggacttc tggtcccaga cccgctctgc
      601 tcaggtgata gtacctcagc aaacaagact ggtcttcgga ccatgccacc cattaacctg
      661 caagagaagc aggtcatctg tctctcagga gatgatagct ccacctgcat tgggattttg
      721 gccaaggagg tggagattgt ggctagcagt gactctagca tttcaagcaa ggcccgggga
      781 agcaacaagg tgaaaattca gcctgtcgcc aagtatgact gggaacagaa gtactactat
      841 ggcaacctga ttgctgtgtc taactccttc ttggcctatg ccattcgggc tgccaacaat
      901 ggctctgcca tggtgcgggt gatcagcgtc agcactt
//
