LOCUS       sequence-117132-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: CYSLTR2-Tango
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatgga aagaaaattt atgtctctgc aaccctctat cagcgtgagc
      301 gaaatggaac ccaatggcac cttctcaaat aataattctc ggaattgcac catcgagaac
      361 tttaaacgag aattcttccc tatcgtgtac ttgattattt ttttttgggg agttctcggc
      421 aatggacttt ctatttacgt cttcctccag ccctacaaaa agtccacatc tgtgaatgtg
      481 tttatgctga atctggcgat ctctgacttg ctctttatca gcacgttgcc ctttagggca
      541 gattactatc ttagaggtag caattggatt tttggagacc tcgcttgtag aattatgagt
      601 tacagcctgt acgtgaacat gtacagcagt atctatttcc tgaccgtgct gtctgtggtc
      661 aggttcctgg ccatggtgca cccattccga ctgttgcatg tcacatccat tagaagcgcc
      721 tggatcttgt gtggcatcat atggatactg ataatggcct cttccattat gcttctggac
      781 agcggttctg agcaaaatgg aagtgtcact agctgcctgg aactgaacct gtataaaatc
      841 gctaaactgc aaacaatgaa ctacattgct cttgtggtgg ggtgtctgct gcctttcttc
      901 acactcagca tctgttacct cctgataatt cgagtattgc tcaaagtgga agtgcccgag
      961 agcggactga gagtgagtca caggaaagcc ctgactacca taattattac gcttatcatt
     1021 ttcttcctgt gcttccttcc atatcacacg ctgcgcactg tgcatctgac aacatggaag
     1081 gtgggtctgt gtaagga
//
