LOCUS       sequence-117140-         462 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sec23B as a fusion with mGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Hughes H, Stephens DJ
  TITLE     Sec16A defines the site for vesicle budding from the endoplasmic 
            reticulum on exit from mitosis.
  JOURNAL   J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8. Epub 2010 Nov 2.
  PUBMED    21045114
REFERENCE   2  (bases 1 to 462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2010-12-1"; volume: "123(Pt 23)"; pages: "4032-8. Epub
            2010 Nov 2"
COMMENT     Sequence Label: pLVXPuro-mGFP-Sec23B
FEATURES             Location/Qualifiers
     source          1..462
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(158..179)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(419..438)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcagatcgcc tggagacgcc atccacgtct gttttgacct ccatagaaga caccgactct
       61 actagaggat cgctagcgct accggactca gatctcgaca ccggtcgcca ccatggtgag
      121 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      181 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      241 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      301 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      361 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      421 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag gg
//
