LOCUS       sequence-117167-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli cloning vector (KanR, low copy, blue/white selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Vieira J, Messing J
  TITLE     New pUC-derived cloning vectors with different selectable markers 
            and DNA replication origins.
  JOURNAL   Gene. 1991 Apr;100:189-94.
  PUBMED    1905257
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1991-04"; volume: "100"; pages: "189-94"
COMMENT     Sequence Label: pOK12
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(11..27)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11..27)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(155..171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(155..171)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(168..190)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    179..195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    250..271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attggggatc gatccactag ttctagagcg gccgccacgg cgatatcgga tccatatgac
       61 gtcgacgcgt ctgcagaagc ttcgaattcg agctcccggg taccatggca tgcatcgata
      121 gatctcgagg cctcggacta gtggcgtaat catggtcata gctgtttcct gtgtgaaatt
      181 gttatccgct cacaattcca cacaacatac gagccgcgga agcataaagt gtaaagcctg
      241 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
      301 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      361 tttgcgtatt ggagctttcg cgagggaagt gagagggccg cggcaaagcc gtttttccat
      421 aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct
      541 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc
      601 acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc
      661 ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga
      721 aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagttt
      781 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
      841 aagccagtta cctcggttca aagagttggt agctca
//
