LOCUS       sequence-117279-         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, 
            Wang Z, Koffas MA
  TITLE     Improvement of catechin production in Escherichia coli through 
            combinatorial metabolic engineering.
  JOURNAL   Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
  PUBMED    25527438
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2014-12-17"; volume: "28C:43-53. doi"; pages: " 
            10.1016/j.ymben.2014.12.002"
COMMENT     Sequence Label: p148
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    64..88
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             103..125
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             280..291
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tccggcgtag cctaggatcg agatcgatct cgatcccgcg aaattaatac gactcactat
       61 aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttaagaa
      121 ggagatatac atatggcacc gaccaccacc ctgaccgcac tggcagaaga aaaaagcctg
      181 cagcagaaat ttgttcgtga tgaagatgaa cgtccgaaag ttgcctataa tgtgtttagc
      241 aatgaaatcc cggttattag cctggcaggt attgatgaaa ttgaaggtcg tcgtagcgaa
      301 atttgccgta aaattgttga agcatgtgaa ggttggggtg tttttcaggt tgttgatcat
      361 ggtgttgatg caaatctgat tgcagaaatg acccgtctgg cacgtgaatt ttttgcactg
      421 cctccggaag aaaaactgcg ttttgatatg agcggtggta aaaaaggtgg ttttattgtt
      481 agcagccatc tgcagggtga agcagttcag gattggcgtg aaattgttac ctatttcagc
      541 tatccgattc gtgcacgtga ttatagccgt tggcctgata aaccggaagg ttggcgtgca
      601 gttaccgaaa cctatagcga aaaactgatg gatctggcat gtaaactgct ggaagttctg
      661 agcgaagcaa tgggtctgga aa
//
