LOCUS       sequence-117297-       11265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP tagged CD47 with its own long 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Berkovits BD, Mayr C
  TITLE     Alternative 3' UTRs act as scaffolds to regulate membrane protein 
            localization.
  JOURNAL   Nature. 2015 Apr 20. doi: 10.1038/nature14321.
  PUBMED    25896326
REFERENCE   2  (bases 1 to 11265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Apr 20. doi: 10.1038/nature14321."
COMMENT     Sequence Label: GFP_CD47_LU
FEATURES             Location/Qualifiers
     source          1..11265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(995..997,998..1000,1001..1711)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1040..1061)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1301..1320)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1648..1669
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     RBS             3406..3417
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(6859..6876)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6865..7089
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7135..7563
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7222..7241)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7432..7453
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7572..7592)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7577..7906
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7757..7892
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7819..7838
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7973..8767
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8027..8046)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8637..8656
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8941..9062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8978..8997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9032..9051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9111..9127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9111..9127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9124..9146)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9135..9151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9159..9165,9166..9183,9184..9189))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9204..9225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9342..9359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9513..10098)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9593..9612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10269..11060,11061..11129))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10892..10911
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11130..11234)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccatgtgg cccctggtag cggcgctgtt
      961 gctgggctcg gcgtgctgcg gatcagctgg atccatggtg agcaagggcg aggagctgtt
     1021 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1081 cgtgtctggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1141 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     1201 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1261 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1321 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1381 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1441 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg
     1501 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1561 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     1621 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1681 gatcactctc ggcatggacg agctgtacaa gcagctacta tttaataaaa caaaatctgt
     1741 agaattcacg ttttgtaatg acactgtcgt cattccatgc tttgttacta atatggaggc
     1801 acaaaacact actgaagtat acgtaaagtg gaaatttaaa ggaagagata tttacacctt
     1861 tgatggagct ctaaacaagt ccactgtccc cactgacttt agtagtgcaa aaattgaagt
     1921 ctcacaatta ctaaaaggag atgcctcttt gaagatggat aagagtgatg ctgtctcaca
     1981 cacaggaaac tacacttgtg aagtaacaga attaaccaga gaaggtgaaa cgatcatcga
     2041 gctaaaatat cgtgttgttt catggttttc tccaaatgaa aatattctta ttgttatttt
     2101 cccaattttt gctatactcc tgttctgggg acagtttggt attaaaacac ttaaatatag
     2161 atccggtggt atggatgaga aaacaattgc tttacttgtt gctggactag tgatcactgt
     2221 cattgtcatt gttggagcca ttcttttcgt cccaggtgaa tattcattaa agaatgctac
     2281 tggccttggt ttaattgtga cttctacagg gatattaata ttacttcact actatgtgtt
     2341 tagtacagcg attggattaa cctccttcgt cattgccata ttggttattc aggtgatagc
     2401 ctatatcctc gctgtggttg gactgagtct ctgtattgcg gcgtgtatac caatgcatgg
     2461 ccctcttctg atttcaggtt tgagtatctt agctctagca caattacttg gactagttta
     2521 tatgaaattt gtggcttcca atcagaagac tatacaacct cctaggaaag ctgtagagga
     2581 accccttaat gcattcaaag aatcaaaagg aatgatgaat gatgaataag cggccgcagt
     2641 gaagtgatgg actccgattt ggagagtagt aagacgtgaa aggaatacac ttgtgtttaa
     2701 gcaccatggc cttgatgatt cactgttggg gagaagaaac aagaaaagta actggttgtc
     2761 acctatgaga cccttacgtg attgttagtt aagtttttat tcaaagcagc tgtaatttag
     2821 ttactcaaat aattatgatc tatgttgttt gcccaattga gatccagttt tttgttgtta
     2881 tttttaatca attaggggca atagtagaat ggacaatttc caagaatgat gcctttcagg
     2941 tcctagggcc tctggcctct aggtaaccag tttaaattgg ttcagggtga taactactta
     3001 gcactgccct ggtgattacc cagagatatc tatgaaaacc agtggcttcc atcaaacctt
     3061 tgccaactca ggttcacagc agctttgggc agttatggca gtatggcatt agctgagagg
     3121 tgtctgccac ttctgggtca atggaataat aaattaagta caggcaggaa tttggttggg
     3181 agcatcttgt atgatctccg tatgatgtga tattgatgga gatagtggtc ctcattcttg
     3241 ggggttgcca ttcccacatt cccccttcaa caaacagtgt aacaggtcct tcccagattt
     3301 agggtacttt tattgatgga tatgttttcc ttttattcac ataacccctt gaaaccctgt
     3361 cttgtcctcc tgttacttgc ttctgctgta caagatgtag caccttttct cctctttgaa
     3421 catggtctag tgacacggta gcaccagttg caggaaggag ccagacttgt tctcagagca
     3481 ctgtgttcac acttttcagc aaaaatagct atggttgtaa catatgtatt cccttcctct
     3541 gatttgaagg caaaaatcta cagtgtttct tcacttcttt tctgatctgg ggcatgaaaa
     3601 aagcaagatt gaaatttgaa ctatgagtct cctgcatggc aacaaaatgt gtgtcaccat
     3661 caggccaaca ggccagccct tgaatgggga tttattactg ttgtatctat gttgcatgat
     3721 aaacattcat caccttcctc ctgtagtcct gcctcgtact ccccttcccc tatgattgaa
     3781 aagtaaacaa aacccacatt tcctatcctg gttagaagaa aattaatgtt ctgacagttg
     3841 tgatcgcctg gagtactttt agacttttag cattcgtttt ttacctgttt gtggatgtgt
     3901 gtttgtatgt gcatacgtat gagataggca catgcatctt ctgtatggac aaaggtgggg
     3961 tacctacagg agagcaaagg ttaattttgt gcttttagta aaaacattta aatacaaagt
     4021 tctttattgg gtggaattat atttgatgca aatatttgat cacttaaaac ttttaaaact
     4081 tctaggtaat ttgccacgct ttttgactgc tcaccaatac cctgtaaaaa tacgtaattc
     4141 ttcctgtttg tgtaataaga tattcatatt tgtagttgca ttaataatag ttatttctta
     4201 gtccatcaga tgttcccgtg tgcctctttt atgccaaatt gattgtcata tttcatgttg
     4261 ggaccaagta gtttgcccat ggcaaaccta aatttatgac ctgctgaggc ctctcagaaa
     4321 actgagcata ctagcaagac agctcttctt gaaaaaaaaa atatgtatac acaaatatat
     4381 acgtatatct atatatacgt atgtatatac acacatgtat attcttcctt gattgtgtag
     4441 ctgtccaaaa taataacata tatagaggga gctgtattcc tttatacaaa tctgatggct
     4501 cctgcagcac tttttccttc tgaaaatatt tacattttgc taacctagtt tgttacttta
     4561 aaaatcagtt ttgatgaaag gagggaaaag cagatggact tgaaaaagat ccaagctcct
     4621 attagaaaag gtatgaaaat ctttatagta aaatttttta taaactaaag ttgtaccttt
     4681 taatatgtag taaactctca tttatttggg gttcgctctt ggatctcatc catccattgt
     4741 gttctcttta atgctgcctg ccttttgagg cattcactgc cctagacaat gccaccagag
     4801 atagtggggg aaatgccaga tgaaaccaac tcttgctctc actagttgtc agcttctctg
     4861 gataagtgac cacagaagca ggagtcctcc tgcttgggca tcattgggcc agttccttct
     4921 ctttaaatca gatttgtaat ggctcccaaa ttccatcaca tcacatttaa attgcagaca
     4981 gtgttttgca catcatgtat ctgttttgtc ccataatatg ctttttactc cctgatccca
     5041 gtttctgctg ttgactcttc cattcagttt tatttattgt gtgttctcac agtgacacca
     5101 tttgtccttt tctgcaacaa cctttccagc tacttttgcc aaattctatt tgtcttctcc
     5161 ttcaaaacat tctcctttgc agttcctctt catctgtgta gctgctcttt tgtctcttaa
     5221 cttaccattc ctatagtact ttatgcatct ctgcttagtt ctattagttt tttggccttg
     5281 ctcttctcct tgattttaaa attccttcta tagctagagc ttttctttct ttcattctct
     5341 cttcctgcag tgttttgcat acatcagaag ctaggtacat aagttaaatg attgagagtt
     5401 ggctgtattt agatttatca ctttttaata gggtgagctt gagagttttc tttctttctg
     5461 tttttttttt ttgttttttt tttttttttt tttttttttt tttttgacta atttcacatg
     5521 ctctaaaaac cttcaaaggt gattattttt ctcctggaaa ctccaggtcc attctgttta
     5581 aatccctaag aatgtcagaa ttaaaataac agggctatcc cgtaattgga aatatttctt
     5641 ttttcaggat gctatagtca atttagtaag tgaccaccaa attgttattt gcactaacaa
     5701 agctcaaaac acgataagtt tactcctcca tctcagtaat aaaaattaag ctgtaatcaa
     5761 ccttctaggt ttctcttgtc ttaaaatggg tattcaaaaa tggggatctg tggtgtatgt
     5821 atggaaacac atactcctta atttacctgt tgttggaaac tggagaaatg attgtcgggc
     5881 aaccgtttat tttttattgt attttatttg gttgagggat ttttttataa acagttttac
     5941 ttgtgtcata ttttaaaatt actaactgcc atcacctgct ggggtccttt gttaggtcat
     6001 tttcagtgac taatagggat aatccaggta actttgaaga gatgagcagt gagtgaccag
     6061 gcagtttttc tgcctttagc tttgacagtt cttaattaag atcattgaag accagctttc
     6121 tcataaattt ctctttttga aaaaaagaaa gcatttgtac taagctcctc tgtaagacaa
     6181 catcttaaat cttaaaagtg ttgttatcat gactggtgag agaagaaaac attttgtttt
     6241 tattaaatgg agcattattt acaaaaagcc attgttgaga attagatccc acatcgtata
     6301 aatatctatt aaccattcta aataaagaga actccagtgt tgctatgtgc aagatcctct
     6361 cttggagctt ttttgcatag caattaaagg tgtgctattt gtcagtagcc atttttttgc
     6421 agtgatttga agaccaaagt tgttttacag ctgtgttacc gttaaaggtt ttttttttta
     6481 tatgtattaa atcaatttat cactgtttaa agctttgaat atctgcaatc tttgccaagg
     6541 tactttttta tttaaaaaaa aacataactt tgtaaatatt accctgtaat attatatata
     6601 cttaataaaa cattttaagc tattttgttg ggctatttct attgctgcta cagcagacca
     6661 caagcacatt tctgaaaaat ttaatttatt aatgtatttt taagttgctt atattctagg
     6721 taacaatgta aagaatgatt taaaatatta attatgaatt ttttgagtat aatacccaat
     6781 aagcttttaa ttagagcaga gttttaatta aaagttttaa atcagtctct agagggcccg
     6841 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     6901 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6961 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     7021 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     7081 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     7141 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7201 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7261 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7321 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7381 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     7441 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7501 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7561 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     7621 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     7681 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     7741 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7801 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     7861 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     7921 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     7981 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     8041 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     8101 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     8161 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     8221 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     8281 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     8341 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     8401 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     8461 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     8521 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     8581 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     8641 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     8701 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     8761 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     8821 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     8881 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     8941 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9001 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9061 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     9121 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9181 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9241 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9301 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9361 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9421 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10021 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc
    10081 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10141 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10201 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10261 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10321 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10381 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10441 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10501 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10561 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10621 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10681 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10741 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10801 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10861 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10921 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10981 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11041 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11101 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11161 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11221 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
