LOCUS       sequence-117299-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans 
            K08F4.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: AP303-4
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(55..77)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_RNA        complement(349..424)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
       61 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
      121 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
      181 atcagagcag attgcactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
      241 aaggagaaaa taccgcatca ggcgccattc gccattcagg atgcgcaact gttgggaagg
      301 gcgatcggtg cgggcctctt cgctattacg ccagaaattt cacaaaaagc accgactcgg
      361 tgccactttt tcaagttgat aacggactag ccttatttta acttgctatt tctagctcta
      421 aaactcttat tctgggttaa ggccaagaca tctcgcaata ggaggtgttt atataggggc
      481 aacacaagag gcggcagaca tctcgtgagt ttgacagagt agtataaaga gtgtgtgtga
      541 gagggtgtag gcatttcata acatattttt tgtggaataa ttttggaaaa aaggtgtttt
      601 tttaataata gcttttataa gagtttctca aaaattgcat gctttttttg aaaaggttga
      661 caaattatag tagtactaaa gttgctattt tcaggtctca gcag
//
