LOCUS       sequence-117315-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express an additional copy of Bem1, integrated at TRP locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Jost AP, Weiner OD
  TITLE     Probing Yeast Polarity with Acute, Reversible, Optogenetic 
            Inhibition of Protein Function.
  JOURNAL   ACS Synth Biol. 2015 Jun 2.
  PUBMED    26035630
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Jun 2."
COMMENT     Sequence Label: pCyc-Bem1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgaccatgat tacgccaagc tcggaattaa ccctcactaa agggaacaaa agctgggccg
       61 gccatttaaa tgtttaaacg gccacgctgg ccatatttct accaatctct caacactgag
      121 taatggtagt tataagaaag agaccgagtt agggacagtt agaggcggtg gagatattcc
      181 ttatggcatg tctggcgatg ataaaacttt tcaaacggca gccccgatct aaaagagctg
      241 acagggaaat ggtcagaaaa agaaacgtgc acccgcccgt ctggacgcgc cgctcacccg
      301 cacggcagag accaatcagt aaaaatcaac ggttaacgac attactatat atataatata
      361 ggaagcattt aatagaacag catcgtaata tatgtgtact ttgcagttat gacgccagat
      421 ggcagtagtg gaagatattc tttattgaaa aatagcttgt caccttacgt acaatcttga
      481 tccggagccc gtcgctttga gagaaatcag aagattccaa aagtctactg aacttttgat
      541 cagaaagttg cctttccaaa gactagtcag agaaatcgcc caagatttca agaccgatct
      601 aagattccaa tcctctgcca tcggtgctct acaggaatcc gttgaagctt acttggtctc
      661 tttgttcgaa gacaccaact tggccgctat ccacgctaag cgtgttacca tccaaaagaa
      721 ggatatcaag ttggctagaa gattgagagg tgaaagatcc taagcacaca gggattgttg
      781 tacaattact ttttatgatt ttagatacac acaaatcttt tctacgatta cctttcattt
      841 attttcgttt catgttttat taatcattct ttttgtatat ttcacatcgg gtggggtttt
//
