LOCUS       sequence-117321-         498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector MBP/hexahistidine tagged variant of the MS2 coat 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Batey RT, Kieft JS
  TITLE     Improved native affinity purification of RNA.
  JOURNAL   RNA. 2007 Aug;13(8):1384-9. Epub 2007 Jun 4.
  PUBMED    17548432
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2007-08"; volume: "13(8)"; pages: "1384-9. Epub 2007 Jun 4"
COMMENT     Sequence Label: pHMM
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     82..105
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             139..150
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
       61 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
      121 gtacccggcc ggggatccat cgagggtagg gcttctaact ttactcagtt cgttctcgtc
      181 gacaatggcg gaactggcga cgtgactgtc gccccaagca acttcgctaa cgggatcgct
      241 gaatggatca gctctaactc gcgttcacag gcttacaaag taacctgtag cgttcgtcag
      301 agctctgcgc agaatcgcaa atacaccatc aaagtcgagg tgcctaaagg cgcctggcgt
      361 tcgtacttaa atatggaact aaccattcca attttcgcca cgaattccga ctgcgagctt
      421 attgttaagg caatgcaagg tctcctaaaa gatggaaacc cgattccctc agcaatcgca
      481 gcaaactccg gcatctac
//
