LOCUS       sequence-117523-         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Fiordalisi JJ, Johnson RL 2nd, Ulku AS, Der CJ, Cox AD
  TITLE     Mammalian expression vectors for Ras family proteins: generation and
            use of expression constructs to analyze Ras family function.
  JOURNAL   Methods Enzymol. 2001;332:3-36. doi: 10.1016/s0076-6879(01)32189-4.
  PUBMED    11305105
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2001"; volume: "332:3-36. doi"; pages: " 
            10.1016/s0076-6879(01)32189-4"
COMMENT     Sequence Label: pCGN R-Ras 38V, 63G
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           106..145
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             175..201
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             181..207
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgggggatct tggtggcgtg
      121 aaactcccgc acctcttcgg ccagcgcctt gtagaagcgc gtatggcttc tagctatcct
      181 tatgacgtgc ctgactatgc cagcctggga ggaccttcta gcggatccca tatgagctct
      241 ggtgctgctt ccggtacagg tcgtggtcgt ccacgtggtg gaggtccagg tcccggggac
      301 cctccgccta gcgagacaca caagcttgtg gtcgtgggtg gtgtcggcgt gggcaagagc
      361 gcgctgacca tccagttcat ccagtcctac ttcgtgtctg actacgaccc cactattggg
      421 gactcctaca cgaagatctg cagtgtggat ggcatcccag cccggctgga catcctggac
      481 accgcgggcc aggaagagtt cggggccatg agagagcagt acatgcgtgc tggccacggc
      541 ttcctgctgg tgttcgccat taatgaccgg cagagtttca acgaggtggg caagctcttc
      601 acgcagattc tgcgggtcaa ggaccgcgac gacttccccg ttgtgttggt cgggaacaag
      661 gcagatctgg agtcacagcg ccaggtcccc cgatcagaag cctctgcctt cggcgcctcc
      721 caccacgtag cctactttga ggcctcggcc aaactgcgtc tcaacgtgga cgaggctttt
      781 gagcagctgg tgcgggctgt ccggaaatac caggaacaag agctcccacc gagccctccc
      841 agtgccccca ggaagaaggg cgggggctgc ccctgcgtcc tcctgtagga tcctgagaac
      901 ttcagggtga gtttggggac ccttgattgt tctttctttt tcgctattg
//
