LOCUS       sequence-117581-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For transposon tagging with YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Sheridan DL, Hughes TE
  TITLE     A faster way to make GFP-based biosensors: two new transposons for 
            creating multicolored libraries of fluorescent fusion proteins.
  JOURNAL   BMC Biotechnol. 2004 Aug 18;4:17.
  PUBMED    15317651
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2004-08-18"; volume: "4"; pages: "17"
COMMENT     Sequence Label: pBNJ28.2
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    16..34
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(104..123)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(105..123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(130..147)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(130..146)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(139..161)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             284..586
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     465..484
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ccttgctggc gcgccagatg tgtataagag acagaagggc gaattctgca gatatccatc
       61 acactggcgg ccgctcgagc atgcatctag agggcccaat tcgccctata gtgagtcgta
      121 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      181 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      241 caccgatcgc ccttcccaac agttgcgcag cctatacgta cggcagttta aggtttacac
      301 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
      361 gccggggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
      421 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
      481 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
      541 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggcatgag
      601 attatcaaaa aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg
      661 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc
      721 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt
//
