LOCUS       sequence-117603-         427 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the T180C mutant of Sulfolobus solfataricus PCNA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Hirakawa H, Kakitani A, Nagamune T
  TITLE     Introduction of selective intersubunit disulfide bonds into 
            self-assembly protein scaffold to enhance an artificial multienzyme 
            complex's activity.
  JOURNAL   Biotechnol Bioeng. 2013 Jul;110(7):1858-64. doi: 10.1002/bit.24861. 
            Epub 2013 Feb 22.
  PUBMED    23404255
REFERENCE   2  (bases 1 to 427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24861";
            journalName: "Biotechnol Bioeng"; date: "2013-07"; volume: "110"; 
            issue: "7"; pages: "1858-64"
COMMENT     Sequence Label: pET15b+PCNA3 T180C
FEATURES             Location/Qualifiers
     source          1..427
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gcagcggcct ggtgccgcgc ggcagccata tgatctacct gaaatctttc gaacgtaaca
       61 tccgcctgat caatatgaaa gtggtttacg atgatgttcg tgtgctgaaa gatattatcc
      121 aggcgctggc ccgcctggtt gatgaagccg tgctgaaatt caaacaggat agcgttgaac
      181 tggtggcact ggatcgtgcg catattagcc tgatctctgt taacctgccg cgcgaaatgt
      241 tcaaagaata cgatgtgaac gatgaattta aattcggttt caacacccag tacctgatga
      301 aaatcctgaa agttgcaaaa cgtaaagaag ccattgaaat cgcaagtgaa agcccggata
      361 gtgtgattat caacattatc ggcagcacca atcgtgaatt taacgttcgc aatctggaag
      421 tgtctga
//
