LOCUS       sequence-117838-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for mFTH1 knock-in via landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBGO
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(613..639)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(685..704)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag tgaattcctc
       61 gagttagctc tcatcaccgt gtcccagggt gtgcttgtca aagagatatt ctgccatgcc
      121 agcttcaggg gcacccatct tgcgtaagtt ggtcacgtgg tcacccagtt ctttaatgga
      181 tttcacctgt tcactcagat aatacgtctc aatgaagtca cataagtggg gatcattctt
      241 gtcagtagcc agtttgtgca gttccagtag tgactgattc acactctttt ccaagtgcag
      301 tgcacactcc attgcattca gcccgctctc ccagtcatca cggtctggtt tctttatatc
      361 ctgcaggaag attcggccac ctcgctggtt ctgcagcttc atcagtttct cggcatgctc
      421 cctctcctca tgagattggt ggagaaagta tttggcaaag ttcttcagag ccacatcatc
      481 tcggtcaaaa taacaagaca tagacagata gacgtaggag gcatacaact ccaggttgat
      541 ctggcggttg atggcagcct ccgcgtcctg gtggtagttc tggcgcactt gcgagggaga
      601 cgcggtggtc atagcgtaat ctggaacatc gtatgggtac atggtggcac tcgaggtcaa
      661 ttctttgcca aaatgatgag acagcacaat aaccagcacg ttgcccagga gctgtaggaa
      721 aaagaagaag gcatgaacat ggttagcaga ggggcccggt ttggactcag agtattttat
      781 cctcatctca aacagtgtat atcattgtaa ccataaagag aaaggcag
//
