LOCUS       sequence-117841-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by 
            mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC42
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             316..465
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     primer_bind     complement(678..695)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gcagaccgtc agatcttcga agagaaggtc gggcctctgg tgggtcggct gcgcctcacg
       61 gcttcgctcc gtcaaaacgg agccaagacc gcgtatcgcg tcaacctaaa actggatcag
      121 gcggacgtcg ttgattccgg acttccgaaa gtgcgctaca ctcaggtatg gtcgcacgac
      181 gtgacaatcg ttgcgaatag caccgaggcc tcgcgcaaat cgttgtacga tttgaccaag
      241 tccctcgtcg cgacctcgca ggtcgaagat cttgtcgtca accttgtgcc gctgggccgt
      301 ggctccggac gagccgatgc tctcgacgat tttgacctcg atatgctggg atccgacgct
      361 ctcgatgact tcgaccttga catgctgggg agtgacgctc tggacgactt cgatctcgac
      421 atgctgggct ccgacgccct ggatgacttc gatctggaca tgctcatcaa ctgacccggg
      481 tcgagcatgc atctagggcg gccaattccg cccctctccc tccccccccc ctaacgttac
      541 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
      601 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
      661 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
      721 agcag
//
