LOCUS       sequence-117881-         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Initial replicative vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pMJ22
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..78
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(93..98,99..116,117..123)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    131..147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     136..158
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     155..171
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     155..171
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     190..210
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        192..210
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    223..330
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     247..263
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     247..263
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(297..313)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(298..314)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      complement(335..582)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     548..566
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
       61 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
      121 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
      181 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc
      241 ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat
      301 accgtcgacc tcgagggggg gcccggtacc ggccgcaaat taaagccttc gagcgtccca
      361 aaaccttctc aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ctgtacagaa
      421 aaaaaagaaa aatttgaaat ataaataacg ttcttaatac taacataact ataaaaaaat
      481 aaatagggac ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg
      541 ggggagggcg tgaatgtaag cgtgacataa ctaattacat gactcgactt aggggcaggg
      601 catgctcatg tagagcgcct gctcgccgtc cgaggcggtg ccgtcgtaca gggcggtgt
//
