LOCUS       sequence-117906-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the wild type of Staphylococcus aureus sortase A in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
  JOURNAL   Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585.
            Epub 2012 Jul 4.
  PUBMED    22729808
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24585";
            journalName: "Biotechnol Bioeng"; date: "2012-12"; volume: "109"; 
            issue: "12"; pages: "2955-61"
COMMENT     Sequence Label: pTH1-SaSrtA
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(13..60)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     56..74
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(139..156)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(172..327)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
ORIGIN
        1 ttctcctttc agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag
       61 ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt tagcagccgg
      121 atccccttcc tgcagtcagt ggtggtggtg gtggtgctcg agtgcggccg cttgaagctg
      181 ccacaaggca ggaacgttgg atggttccca tcctgccaag gaggtgtggg gctgcaaaca
      241 tttatacgtc ttgccgttat atgtgaccaa ttgtcccgca gtataagctg tgttgacctg
      301 ccaagcggat acaccaggat ttgtcgtgag gcctgagttc agaccggtga ggccagtagc
      361 gtggctgacg aacccgttcg tgataaacgc gtggtctgcc gtgtcgacac gaaggctata
      421 caccggctgc acgccggcgt cggtgacact ggcgactttc gcgtagtaga accgcccgtc
      481 ggtcagctcg tcggcgatag cttgggcgtc cgggtctcgg tggtgtgctt ccaagaaacg
      541 caccagtcca gggacgccga ctgtgtaggt tgtgggcgca aattcgggtt tcccgcgggc
      601 aaaacctgca cagtcgacgc tgaatgcgct gcgttgaatc accgcgtaat cgcccggctt
      661 gatttcgtcg atcagcttcc acagcagggt cggcaccccg gcgacgtcga ccaaacacaa
      721 caacgggtgg ttcgcggtgc ccgtcacacg cagaccttcg accgtacgca ccgtgtacac
      781 cggatgctcg ccggagtgga acagccggtc ggcgagcacg ggattgccat gccggtcaag
      841 gactttcagg tcgatggcgt tgtcactgtt gggccgcgc
//
