LOCUS       sequence-117978-        6449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Derivative of pSiM24 plant expression vector without the M24 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Sahoo DK, Dey N, Maiti IB
  TITLE     pSiM24 is a novel versatile gene expression vector for transient 
            assays as well as stable expression of foreign genes in plants.
  JOURNAL   PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988.
            eCollection 2014.
  PUBMED    24897541
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-06-4"; volume: "9(6):e98988. doi"; pages: " 
            10.1371/journal.pone.0098988. eCollection 2014"
COMMENT     Sequence Label: pSi
FEATURES             Location/Qualifiers
     source          1..6449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      59..700
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        complement(1780..1959)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    2194..2218
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2333..2921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2413..2432)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3092..3883,3884..3952))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3715..3734
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3953..4057)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4098..4708
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4185..4716
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    6287..6311
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttggat ccctcgaggt taacacgcgt gtcgacgagc tcctgcagga tcctctagag
       61 ctttcgttcg tatcatcggt ttcgacaacg ttcgtcaagt tcaatgcatc agtttcattg
      121 cgcacacacc agaatcctac tgagttcgag tattatggca ttgggaaaac tgtttttctt
      181 gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg
      241 tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa
      301 attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat
      361 atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt
      421 aatatgagga gtaaaacact tgtagttgta ccattatgct tattcactag gcaacaaata
      481 tattttcaga cctagaaaag ctgcaaatgt tactgaatac aagtatgtcc tcttgtgttt
      541 tagacattta tgaactttcc tttatgtaat tttccagaat ccttgtcaga ttctaatcat
      601 tgctttataa ttatagttat actcatggat ttgtagttga gtatgaaaat attttttaat
      661 gcattttatg acttgccaat tgattgacaa catgcatcaa tcgatactag tctagtaaca
      721 tagatgacac cgcgagcgat aatttatcct agtttgcgag ctatattttg ttttctatcg
      781 cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca
      841 tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca
      901 tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat
      961 tcagaagaac tcatcgagga gcctgtagaa agcaatcctt tgggaatctg gagcagcaat
     1021 tccgtaaagc acaaggaatc tatcagccca ctctccacca agttcttcag caatatccct
     1081 agtagcgaga gcaatatcct gatacctatc agcaactcca agccttccgc aatcaatgaa
     1141 tccagaaaac cttccgttct ccaccataat gtttggaagg caagcatcac catgagtaac
     1201 aacaagatcc tcaccatctg gcattctagc cttgagccta gcaaaaagtt cagctggagc
     1261 caatccttga tgttcctcat caagatcatc ctgatccaca agtccagctt ccatcctagt
     1321 tctagccctt tcaattctgt gcttagcttg gtgatcaaat gggcaagtag caggatcaag
     1381 agtgtgaagc cttctcatag catcagccat aatggaaacc ttctcagctg gagcaaggtg
     1441 tgaagaaagc aaatcttgtc ctggaacctc tcccaaaaga agccaatctc ttccagcctc
     1501 agtaacaaca tcaagaacag cagcgcatgg aacaccagta gtagcaagcc aagaaagtct
     1561 agcagcctca tcttgaagct cattaagagc accagaaaga tcagtcttaa cgaaaagaac
     1621 tggccttccc tgagcagaaa gcctaaaaac agcagcatca gagcatccaa tagtctgttg
     1681 agcccaatcg tatccaaaaa gcctctcaac ccaagcagca ggacttccag catgaagtcc
     1741 atcttgctca attcccattt gagagtgaat atgagactct aattggatac cgaggggaat
     1801 ttatggaacg tcagtggagc atttttgaca agaaatattt gctagctgat agtgacctta
     1861 ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt gcttagctca ttaaactcca
     1921 gaaacccgcg gctcagtggc tccttcaacg ttgcggttct gtcagttcca aacgtaaaac
     1981 ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga
     2041 tcagatctgg tgacccctat gtaaggggcc ggccatttaa attcgcgatt aattaacctg
     2101 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
     2161 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
     2221 cactgatagt ttaaactgaa ggcgggaaac gacaatctga tatcgcgcgc ggtacctgag
     2281 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2341 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2401 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2461 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2521 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2581 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2641 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2701 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2761 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2821 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2881 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2941 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3001 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3061 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3121 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3181 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagaaccac
     3241 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3301 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3361 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3421 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3481 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3541 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3601 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3661 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3721 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3781 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3841 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3901 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3961 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4021 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4081 gggccccgct caccgggctg gttgccctcg ccgctgggct ggcggccgtc tatggccctg
     4141 caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca ccgcggccgc cggcgttgtg
     4201 gatacctcgc ggaaaacttg gccctcactg acagatgagg ggcggacgtt gacacttgag
     4261 gggccgactc acccggcgcg gcgttgacag atgaggggca ggctcgattt cggccggcga
     4321 cgtggagctg gccagcctcg caaatcggcg aaaacgcctg attttacgcg agtttcccac
     4381 agatgatgtg gacaagcctg gggataagtg ccctgcggta ttgacacttg aggggcgcga
     4441 ctactgacag atgaggggcg cgatccttga cacttgaggg gcagagtgct gacagatgag
     4501 gggcgcacct attgacattt gaggggctgt ccacaggcag aaaatccagc atttgcaagg
     4561 gtttccgccc gtttttcggc caccgctaac ctgtctttta acctgctttt aaaccaatat
     4621 ttataaacct tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg
     4681 ggggtgcccc cccttctcga accctcccgg cccgctgtcg actacctaga tgtggcgcaa
     4741 cgatgccggc gacaagcagg agcgcaccga cttcttccgc atcaagtgtt ttggctctca
     4801 ggccgaggcc cacggcaagt atttgggcaa ggggtcgctg gtattcgtgc agggcaagat
     4861 tcggaatacc aagtacgaga aggacggcca gacggtctac gggaccgact tcattgccga
     4921 taaggtggat tatctggaca ccaaggcacc aggcgggtca aatcaggaat aagggcacat
     4981 tgccccggcg tgagtcgggg caatcccgca aggagggtga atgaatcgca ccttcgatcg
     5041 caaggcgtat cgccaggaac tgatcgacgc cggcttctcg gccgaagatg ccgaaacgat
     5101 cgcctcgcgc accgtcatgc gtgccccgcg cgaaaccttc cagtcggtcg gctcgatggt
     5161 ccagcaggcc accgccaaga tcgaacgcga ttcggtccag ctggccccgc cggcccttcc
     5221 ggccccgtcg gccgccgtcg aacgctcgcg ccggctggaa caggaagccg ccggcctggc
     5281 caagtcgatg accatcgaca cccgcggcac catgaccacc aagaagcgca agaccgccgg
     5341 cgaggatctg gccaagcagg tcagcgaagc caagcaggcc gccctgctga agcataccaa
     5401 gcagcagatc aaggaaatgc agctgtcgct gttcgacatc gccccgtggc cggataccat
     5461 gcgggccatg ccgaatgata ccgcccgctc ggccctgttc accacccgca ataagaagat
     5521 cccgcgcgaa gccctccaga ataaggtcat cttccacgtc aacaaggatg tcaagatcac
     5581 ctataccggc gtcgaactgc gggccgatga tgatgaactg gtctggcagc aggtcctgga
     5641 atatgccaag cgcaccccga tcggcgaacc gatcaccttc accttctatg aactgtgcca
     5701 ggatctgggc tggtcgatca atggccgcta ttataccaag gccgaagaat gcctgtcgcg
     5761 cctccaggcg accgcgatgg gcttcacctc ggatcgcgtc ggccatctgg aatcggtcag
     5821 cctgctgcac cgcttccgcg tcctggatcg cggcaagaag acctcgcgct gccaggtcct
     5881 gatcgacgaa gaaatcgtcg tcctgttcgc cggcgatcat tataccaagt tcatctggga
     5941 gaagtatcgc aagctgtcgc cgaccgcccg ccgcatgttc gattatttct cgtcgcaccg
     6001 cgaaccgtat ccgctgaagc tggaaacctt ccgcctgatg tgcggctcgg attcgacccg
     6061 cgtcaagaag tggcgcgaac aggtcggcga agcctgcgaa gaactgcgcg gctcgggcct
     6121 ggtcgaacat gcctgggtca atgatgatct ggtccattgc aagcgctagg gccttgtggg
     6181 gtcagttccg gctgggggtt cagccagcgc tttacgatat cgcgcgcggt acccagtaca
     6241 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     6301 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     6361 tcgatacagg cagcccatca gtccactaga ccagtacctt ggaggcctgt gcgcatccca
     6421 gggtgggtcc cgtatacccc ggggaattc
//
