LOCUS       sequence-117982-        7063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant Gene expression Vector pSIM24 containing eGFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Sahoo DK, Dey N, Maiti IB
  TITLE     pSiM24 is a novel versatile gene expression vector for transient 
            assays as well as stable expression of foreign genes in plants.
  JOURNAL   PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988.
            eCollection 2014.
  PUBMED    24897541
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-06-4"; volume: "9(6):e98988. doi"; pages: " 
            10.1371/journal.pone.0098988. eCollection 2014"
COMMENT     Sequence Label: pSiM24-eGFP
FEATURES             Location/Qualifiers
     source          1..7063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      23..664
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        complement(1744..1923)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    2158..2182
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2297..2885)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2377..2396)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3056..3847,3848..3916))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3679..3698
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3917..4021)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4062..4672
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4149..4680
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    6251..6275
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gagctcctgc aggatcctct agagctttcg ttcgtatcat cggtttcgac aacgttcgtc
       61 aagttcaatg catcagtttc attgcgcaca caccagaatc ctactgagtt cgagtattat
      121 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
      181 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
      241 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
      301 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
      361 tcgtggcctc taatgaccga agttaatatg aggagtaaaa cacttgtagt tgtaccatta
      421 tgcttattca ctaggcaaca aatatatttt cagacctaga aaagctgcaa atgttactga
      481 atacaagtat gtcctcttgt gttttagaca tttatgaact ttcctttatg taattttcca
      541 gaatccttgt cagattctaa tcattgcttt ataattatag ttatactcat ggatttgtag
      601 ttgagtatga aaatattttt taatgcattt tatgacttgc caattgattg acaacatgca
      661 tcaatcgata ctagtctagt aacatagatg acaccgcgag cgataattta tcctagtttg
      721 cgagctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
      781 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
      841 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
      901 tttattgcca aatgtttgaa cgattcagaa gaactcatcg aggagcctgt agaaagcaat
      961 cctttgggaa tctggagcag caattccgta aagcacaagg aatctatcag cccactctcc
     1021 accaagttct tcagcaatat ccctagtagc gagagcaata tcctgatacc tatcagcaac
     1081 tccaagcctt ccgcaatcaa tgaatccaga aaaccttccg ttctccacca taatgtttgg
     1141 aaggcaagca tcaccatgag taacaacaag atcctcacca tctggcattc tagccttgag
     1201 cctagcaaaa agttcagctg gagccaatcc ttgatgttcc tcatcaagat catcctgatc
     1261 cacaagtcca gcttccatcc tagttctagc cctttcaatt ctgtgcttag cttggtgatc
     1321 aaatgggcaa gtagcaggat caagagtgtg aagccttctc atagcatcag ccataatgga
     1381 aaccttctca gctggagcaa ggtgtgaaga aagcaaatct tgtcctggaa cctctcccaa
     1441 aagaagccaa tctcttccag cctcagtaac aacatcaaga acagcagcgc atggaacacc
     1501 agtagtagca agccaagaaa gtctagcagc ctcatcttga agctcattaa gagcaccaga
     1561 aagatcagtc ttaacgaaaa gaactggcct tccctgagca gaaagcctaa aaacagcagc
     1621 atcagagcat ccaatagtct gttgagccca atcgtatcca aaaagcctct caacccaagc
     1681 agcaggactt ccagcatgaa gtccatcttg ctcaattccc atttgagagt gaatatgaga
     1741 ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat
     1801 atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt
     1861 atgtgcttag ctcattaaac tccagaaacc cgcggctcag tggctccttc aacgttgcgg
     1921 ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg
     1981 actcccttaa ttctccgctc atgatcagat ctggtgaccc ctatgtaagg ggccggccat
     2041 ttaaattcgc gattaattaa cctgtggttg gcatgcacat acaaatggac gaacggataa
     2101 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt
     2161 tacccgccaa tatatcctgt caaacactga tagtttaaac tgaaggcggg aaacgacaat
     2221 ctgatatcgc gcgcggtacc tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2281 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2341 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2401 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2461 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2521 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2581 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2641 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2701 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2761 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2821 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2881 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2941 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3001 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3061 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3121 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3181 ctgcaatgat accgcgagaa ccacgctcac cggctccaga tttatcagca ataaaccagc
     3241 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3301 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3361 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3421 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3481 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3541 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3601 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3661 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3721 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3781 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3841 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3901 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3961 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4021 gcacatttcc ccgaaaagtg ccacgggccc cgctcaccgg gctggttgcc ctcgccgctg
     4081 ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga
     4141 gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc actgacagat
     4201 gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg acagatgagg
     4261 ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg
     4321 cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata agtgccctgc
     4381 ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc ttgacacttg
     4441 aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg ctgtccacag
     4501 gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc taacctgtct
     4561 tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc tgcgccctgt
     4621 gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc ccggcccgct
     4681 gtcgactacc tagatgtggc gcaacgatgc cggcgacaag caggagcgca ccgacttctt
     4741 ccgcatcaag tgttttggct ctcaggccga ggcccacggc aagtatttgg gcaaggggtc
     4801 gctggtattc gtgcagggca agattcggaa taccaagtac gagaaggacg gccagacggt
     4861 ctacgggacc gacttcattg ccgataaggt ggattatctg gacaccaagg caccaggcgg
     4921 gtcaaatcag gaataagggc acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg
     4981 gtgaatgaat cgcaccttcg atcgcaaggc gtatcgccag gaactgatcg acgccggctt
     5041 ctcggccgaa gatgccgaaa cgatcgcctc gcgcaccgtc atgcgtgccc cgcgcgaaac
     5101 cttccagtcg gtcggctcga tggtccagca ggccaccgcc aagatcgaac gcgattcggt
     5161 ccagctggcc ccgccggccc ttccggcccc gtcggccgcc gtcgaacgct cgcgccggct
     5221 ggaacaggaa gccgccggcc tggccaagtc gatgaccatc gacacccgcg gcaccatgac
     5281 caccaagaag cgcaagaccg ccggcgagga tctggccaag caggtcagcg aagccaagca
     5341 ggccgccctg ctgaagcata ccaagcagca gatcaaggaa atgcagctgt cgctgttcga
     5401 catcgccccg tggccggata ccatgcgggc catgccgaat gataccgccc gctcggccct
     5461 gttcaccacc cgcaataaga agatcccgcg cgaagccctc cagaataagg tcatcttcca
     5521 cgtcaacaag gatgtcaaga tcacctatac cggcgtcgaa ctgcgggccg atgatgatga
     5581 actggtctgg cagcaggtcc tggaatatgc caagcgcacc ccgatcggcg aaccgatcac
     5641 cttcaccttc tatgaactgt gccaggatct gggctggtcg atcaatggcc gctattatac
     5701 caaggccgaa gaatgcctgt cgcgcctcca ggcgaccgcg atgggcttca cctcggatcg
     5761 cgtcggccat ctggaatcgg tcagcctgct gcaccgcttc cgcgtcctgg atcgcggcaa
     5821 gaagacctcg cgctgccagg tcctgatcga cgaagaaatc gtcgtcctgt tcgccggcga
     5881 tcattatacc aagttcatct gggagaagta tcgcaagctg tcgccgaccg cccgccgcat
     5941 gttcgattat ttctcgtcgc accgcgaacc gtatccgctg aagctggaaa ccttccgcct
     6001 gatgtgcggc tcggattcga cccgcgtcaa gaagtggcgc gaacaggtcg gcgaagcctg
     6061 cgaagaactg cgcggctcgg gcctggtcga acatgcctgg gtcaatgatg atctggtcca
     6121 ttgcaagcgc tagggccttg tggggtcagt tccggctggg ggttcagcca gcgctttacg
     6181 atatcgcgcg cggtacccag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     6241 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     6301 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccac tagaccagta
     6361 ccttggaggc ctgtgcgcat cccagggtgg gtcccgtata ccccggggaa ttcgtcaact
     6421 tcgtccacag acatcaacat cttatcgtcc tttgaagata agataataat gttgaagata
     6481 agagtgggag ccaccactaa aacattgctt tgtcaaaagc taaaaaagat gatgcccgac
     6541 agccacttgt gtgaagcatg tgaagccggt ccctccacta agaaaattag tgaagcatct
     6601 tccagtggtc cctccactca cagctcaatc agtgagcaac aggacgaagg aaatgacgta
     6661 agccatgacg tctaatcccc ccaacttcgt ccacagacat caacatctta tcgtcctttg
     6721 aagataagat aataatgttg aagataagag tgggagccac cactaaaaca ttgctttgtc
     6781 aaaagctaaa aaagatgatg cccgacagcc acttgtgtga agcatgtgaa gccggtccct
     6841 ccactaagaa aattagtgaa gcatcttcca gtggtccctc cactcacagc tcaatcagtg
     6901 agcaacagga cgaaggaaat gacgtaagcc atgacgtcta atcccacaag aatttcctta
     6961 tataaggaac acaaatcaga aggaagagat caatcgaaat caaaatcgga atcgaaatca
     7021 aaatcggaat cgaaatctct catctaagct tggatccctc gag
//
