LOCUS       sequence-118007-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArf1 with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARF1-V5-His6
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(60..101)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    121..145
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(707..731)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    772..796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(796..815)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(797..815)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(899..918)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatcaaact caatggtgat ggtgatgatg accggtacgc
       61 gtagaatcga gaccgaggag agggttaggg ataggcttac cttcaagctt cgaattgatc
      121 accactttgt acaagaaagc tgggtccttc tggttccgga gctgattgga cagccagtcc
      181 agtccttcat agagcccgtc gccgctggtg gcgcaggtgg cctgaatgta ccagttcctg
      241 tggcgtagtg agtgcagccc cagcttgtct gtgatctcgg ccgcattcat ggcgttgggg
      301 aggtcctgct tgttggcgaa caccaggagg acagcatccc ggagctcgtc ctcggccagc
      361 atcctcatga gctcctcacg ggcctcgttc acacgctctc tgtcattgct gtccaccacg
      421 aagatcaggc cttgtgtgtt ctggaagtag tggcgccaca ggggccggat cttgtcctgg
      481 ccacccacgt cccacacagt gaagctgatg ttcttgtact ccacggtttc cacgttgaag
      541 cctatggtgg gaatggtggt cacgatctca cccagcttaa gcttgtagag gatcgtggtc
      601 ttccctgcag catccaggcc caccatgagg atgcgcattt cttttttgcc aaaaaggccc
      661 ttgaagaggt tggcgaagat gttccccatg gttctatctc cttctaagcc tgcttttttg
      721 tacaaacttg tgatattcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt
      781 atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc
      841 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
      901 tatatcgccg acatcaccga tgg
//
