LOCUS       sequence-118118-        8984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blue light dependent photoswitching system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Kawano F, Suzuki H, Furuya A, Sato M
  TITLE     Engineered pairs of distinct photoswitches for optogenetic control 
            of cellular proteins.
  JOURNAL   Nat Commun. 2015 Feb 24;6:6256. doi: 10.1038/ncomms7256.
  PUBMED    25708714
REFERENCE   2  (bases 1 to 8984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-02-24"; volume: "6:6256. doi"; pages: " 
            10.1038/ncomms7256"
COMMENT     Sequence Label: pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP
FEATURES             Location/Qualifiers
     source          1..8984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2102..2551
                     /codon_start=1
                     /product="""positive Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=pMagFast2
                     /note="rapidly heterodimerizes with nMagHigh1 upon blue 
                     light irradiation to form a complex with fast switch-off 
                     kinetics"
                     /translation="HTLYAPGGYDIMGYLRQIRNRPNPQVELGPVDTSCALVLCDLKQK
                     DTPVVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
     primer_bind     complement(4578..4595)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4584..4808
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4854..5282
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4941..4960)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5151..5172
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5291..5311)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5296..5625
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5476..5611
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5538..5557
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5692..6486
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5746..5765)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6356..6375
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6660..6781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6697..6716)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6751..6770
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6830..6846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6830..6846)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6843..6865)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6854..6870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6878..6884,6885..6902,6903..6908))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6923..6944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7061..7078)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7232..7817)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7312..7331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7988..8779,8780..8848))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8611..8630
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8849..8953)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgcgaca gctgtcggat gcggataagc tgcgcaaggt
      961 gatctgtgaa ctgctggaga ctgaacgcac ctatgtgaaa gacttaaact gcctcatgga
     1021 gagatacctg aagccccttc agaaggagac ctttctcacc caggatgagc ttgatgtact
     1081 ttttggaaat ttaaccgaaa tggtggagtt tcaagtcgag ttccttaaga ctctggaaga
     1141 tggagtaaga ctggtccctg acttggaaaa gctggagaag gttgaccagt tcaagaaagt
     1201 gctcttctct ctggggggct ccttcctgta ctacgcggac cgcttcaagc tctacagtgc
     1261 cttctgtgcg agccacacaa aagtccccaa ggtcctggtg aaagccaaga cggacacagc
     1321 cttcaaggcg ttcctcgatg cccagaaccc gaggcagcag cactcatcca cgctggagtc
     1381 ttacctcatc aagcccatcc agagggtcct caagtacccg cttctgctca gggagctgtt
     1441 tgcgctgacc gatgcggaga gcgaggaaca ctaccacctg gacgtggcca tcaagactat
     1501 gaacaaggtc gccagtcaca tcaatgagat gcagaagatc cacgaagagt ttggtgccgt
     1561 gtttgaccag ctgattgctg agcagacggg agagaagaaa gaggttgcag atctgagcat
     1621 gggtgacctg ctcttgcaca ccagcgtcat ctggctgaac ccacccgcct cactgggaaa
     1681 gtggaaaaag gagccagaat tagcagcctt cgtcttcaaa acggccgtgg tccttgtata
     1741 taaagacggt tccaagcaga agaagaaact tgttggctct cacaggctgt caatctacga
     1801 ggagtgggac cctttccggt ttcgccacat gatccctacc gaagctttgc aggtccgagc
     1861 tctgcccagt gcagatgcag aggcaaatgc ggtatgcgaa attgtccacg tgaaatcaga
     1921 gtcagaaggg aggcccgagc gggttttcca cctctgctgc agttccccag agagcagaaa
     1981 ggactttctg aagtctgtgc attcgatcct gcgagataaa cacagaagac agctcctcaa
     2041 aacggaagga tccggcggct ccggaggcag cggcggttct ggaggttccg gtggcggctc
     2101 ccacactctt tacgcccctg gaggatacga cattatggga tatttgcggc agattaggaa
     2161 ccgcccaaac cctcaggtcg aactggggcc tgtggacacg tcatgtgccc tggtcctgtg
     2221 cgatctgaag caaaaggaca ctccggtggt ctacgcctcg gaagccttct tgtatatgac
     2281 cggatacagc aatgcagagg tgctcggcag gaactgcaga ttcctgcagt cccccgacgg
     2341 gatggtgaaa ccaaagtcga ctcgcaaata tgtggactcg aacacgatca acaccatgcg
     2401 gaaggccatc gaccggaacg ccgaggtcca ggtggaggtg gtcaacttta agaagaacgg
     2461 ccagcggttc gtgaactttc tgaccatgat tccggtccgg gatgaaaccg gagagtacag
     2521 atactccatg ggattccagt gcgaaaccga aggttccggc ggctcgggcg gatcaggggg
     2581 aagcggacac acactctacg ccccgggagg ctacgatatc atgggctacc tgaggcagat
     2641 ccggaacaga ccgaaccccc aagtggaact cggccccgtg gacacttctt gcgctctggt
     2701 gctgtgcgac ctgaagcaga aggatacccc cgtggtgtac gcatccgaag cgttcctcta
     2761 catgaccggg tactcgaacg cggaggtgct gggtcgcaac tgccggttcc tgcagagccc
     2821 cgacggaatg gtcaagccca agtccactcg gaaatacgtg gattccaaca ccatcaatac
     2881 tatgaggaag gctatcgata ggaacgcgga agtccaagtg gaggtggtga acttcaagaa
     2941 gaatggccag agattcgtca atttccttac catgatccct gtgcgcgatg agactggaga
     3001 gtaccgatac tcaatgggct tccagtgtga aaccgaggga agcggcggtt ccggggggtc
     3061 gggaggttcc ggacataccc tctacgcgcc ggggggttat gacatcatgg gttacctcag
     3121 acagatcaga aaccggccga acccacaagt ggagctggga cccgtcgaca cctcctgcgc
     3181 cctcgtgctg tgtgacctta agcagaagga cacccctgtg gtgtacgcct ccgaagcatt
     3241 cctgtacatg accgggtact cgaacgccga agtgctggga cggaactgcc gcttcctgca
     3301 atccccggat ggaatggtga agcctaagtc aacccgcaaa tacgtggact ccaacactat
     3361 caacaccatg cgcaaggcca ttgaccgcaa tgctgaggtg caagtggaag tggtgaactt
     3421 caagaagaac ggacagcgct tcgtcaactt cctgactatg attcccgtgc gggacgaaac
     3481 cggcgaatac cggtacagca tggggtttca gtgcgagact gagggctccg gcggcagcgg
     3541 tggctccgga ggcagcggag gtaccggcgg cagtggaggg tctggtggca gcggaatggc
     3601 cgaagggagt gtcgccagac agcctgacct gctgacttgc gatgacgaac caatccacat
     3661 tcccggcgct atccagcccc acggactgct gctggctctg gccgctgaca tgactatcgt
     3721 ggcaggctct gataacctgc cagagctgac cgggctggct atcggagcac tgattggccg
     3781 gtccgcagcc gacgtgttcg attctgagac tcacaataga ctgaccatcg ccctggctga
     3841 acctggagcc gccgtgggag ccccaattac cgtcggcttc acaatgcgaa aggacgctgg
     3901 ctttatcggg tcctggcacc gccatgatca gctgattttt ctggagctgg aaccacctca
     3961 gagagacgtg gcagagccac aggctttctt tcggagaaca aacagcgcca tccggcggct
     4021 ccaggccgct gagactctgg aatccgcttg cgcagccgct gcacaggaag tgaggaagat
     4081 caccgggttc gatcgcgtca tgatctaccg gttcgcctca gactttagcg gagaagtgat
     4141 cgcagaagat cgctgtgccg aggtcgaaag taaactgggc ctgcattacc cagcctcaac
     4201 cgtgcctgca caggcacgac ggctgtatac aatcaaccca gtccggatca ttcccgacat
     4261 taattacaga ccagtgcccg tcacacctga cctgaaccca gtgactggga ggcccatcga
     4321 cctgagcttc gccattctgc gcagcgtgtc ccctgtccac ctggagttta tgaggaatat
     4381 cggaatgcat ggcaccatgt ctatcagtat tctgagaggc gaaaggctgt gggggctgat
     4441 cgtgtgccac catcggacac cttactatgt ggacctggac ggcaggcagg cttgtgaact
     4501 ggtcgctcag gtgctggctt ggcagattgg cgtgatggaa gagtaatcta gagggcccgt
     4561 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     4621 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     4681 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4741 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4801 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     4861 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4921 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4981 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5041 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5101 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5161 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5221 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5281 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5341 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5401 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5461 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5521 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     5581 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     5641 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     5701 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     5761 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5821 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5881 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5941 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     6001 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     6061 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     6121 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     6181 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     6241 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     6301 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     6361 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     6421 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     6481 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     6541 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     6601 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     6661 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6721 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6781 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6841 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6901 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6961 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7021 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7081 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7141 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7201 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7261 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7321 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7381 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7441 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7501 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7561 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7621 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7681 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7741 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     7801 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7861 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7921 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7981 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8041 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8101 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8161 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8221 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8281 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8341 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8401 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8461 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8521 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8581 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8641 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8701 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8761 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8821 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8881 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8941 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
