LOCUS       sequence-118170-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl1 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL1
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    64..88
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(656..680)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(712..731)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(713..731)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(776..978)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(805..825)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttcacaagaa ttgggacaac tccagtgaaa agttcttctc ctttgctagc cattctagat
       61 cgaaccactt tgtacaagaa agctgggtcc tattactgtc tgctttttaa tgtttcaact
      121 aaccattcca ttgcctcatc aaggccggtg cctttggttg ctgacgtttt gaatatctgc
      181 cattttcggt ccttcaaggc aggtaaccca agtgaatttg ccatctctga ggaagtcatg
      241 gcctgttcca tgtcctgttt atttgcaaac accactaaaa tggcttttct cagctcttct
      301 tcctccaaca tggcaactaa ctctgatttg gaaatgccaa ttcggtctcg gtcacaactg
      361 tctactacat aaatgactgc atctgtgttt gaatagtaac atctccaata tggcctgata
      421 cttgtctgtc ctcctaaatc ccagacttgg aatttaaggt ttttgtacgt caccgtctct
      481 acattaaatc caatggtagg tatagtagta acaacttctc ccacttgtaa tctgtacaaa
      541 attgtggttt ttcctgctcc atctaatccc aaaattaaaa ttctcatttc ccgagttcca
      601 aacagactgg aaaatatact tgagaaaaag ccacccatgg ttctatctcc ttctaagcct
      661 gcttttttgt acaaacttgt ttgatcaagc ttaactagcc agcttgggtc tccctatagt
      721 gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc
      781 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
      841 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
      901 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccatggatgt
      961 actgccaaac cgcatcacca tggtaatagc gatgactaa
//
