LOCUS       sequence-118175-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gltA-udhA targeting guide RNA array E. coli , Low Phosphate 
            Induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     SUPERSEDE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCASCADE-gltA2-udhA
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(42..144)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(174..800)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     705..724
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
       61 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
      121 ttggaacctc ttacgtgccg atcaacgtct cattttcgcc agatatcgac gtcttaagac
      181 ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag gccgaataag
      241 aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa taatggcggc
      301 atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc ttgatcttcc
      361 aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc attctctagt
      421 gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata ctgtttttct
      481 gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa agcacatcta
      541 aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa agggcaaaag
      601 tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc ccgcttattt
      661 tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag tttacgggtt
      721 gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat cactttactt
      781 ttatctaatc tagacatcat ccaggcatca
//
