LOCUS       sequence-118200-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl8b with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL8B
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(663..687)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(719..738)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(720..738)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(783..882)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(812..832)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aattgggaca actccagtga aaagttcttc tcctttgcta gccattctag atcgaaccac
       61 tttgtacaag aaagctgggt cctatcagct tcttctagat tttgaatgct gaataagcca
      121 ctgaagtgtg atatctatat tatccttttc tttgcaagaa attgaatagc agcaaatttc
      181 tctatcctga atagcagaca gattcatttt ttcaattagc tgtttctcat ccaaggcatt
      241 aggaagatct ctcttgtttc caagcactag cactggaatt ccttgtaact gtggtttatc
      301 tagaagatta tgtagctcat ttcgggaagc ttctatcttt tcacgatctg cagcatctat
      361 catgtaaaca atagcattga ctcctctgca ataccgctcc cacatgcttc gaaatcgggg
      421 ttgtcctcct atgtcccaga tctttattgt gacgttacct ttagttacct tcctcatgtt
      481 gaagcccact gtgggtatca tatcttcact gaattgacct gacgcgatga cattgacgaa
      541 ggtggtcttg cccgagtact gcagccccac gagcgtcagc tccatctctt ccttccagaa
      601 gagcgaacgg aaccagtcca gcaggcggga gatgagcgcc agcatggttc tatctccttc
      661 taagcctgct tttttgtaca aacttgtttg atcaagctta actagccagc ttgggtctcc
      721 ctatagtgag tcgtattaat ttcgataagc cagtaagcag tgggttctct agttagccag
      781 agagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
      841 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
      901 ttgac
//
