LOCUS       sequence-118221-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic 
            configuration with G6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Jones JA, Vernacchio VR, Lachance DM, Lebovich M, Fu L, Shirke AN, 
            Schultz VL, Cress B, Linhardt RJ, Koffas MA
  TITLE     ePathOptimize: A Combinatorial Approach for Transcriptional 
            Balancing of Metabolic Pathways.
  JOURNAL   Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301.
  PUBMED    26062452
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-11"; volume: "5:11301. doi"; pages: " 
            10.1038/srep11301"
COMMENT     Sequence Label: pETM6-G6-vioABECD
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..68)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     64..82
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(121..165)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tcgactcctc ctttcgctag caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt
       61 tatgctagtt attgctcagc ggtggcagca gtttaggtta attaagctgc gactagtaga
      121 cgagtccatg tgctggcgtt caaatttcgc agcagcggtt tctttaccag actcgagtta
      181 acgttgcagc gcataaccta atgcctttgc tagcgcttct ggtaaagaag gcagctgctc
      241 acgacgttca gtaaaacttt gagcgatttg agaagtactt agatgcatta gctctcctac
      301 actttcaaac caaaggcgac ttgtttgtgc atgttgatca aacaatttca tgacaggcat
      361 cactcgcgta ttaaagtgct ctaccacagc aatgacgtta tttgcatgtt gatggacaga
      421 tttaatcagt aactgtgcgc caaccactgc catggtcgtg ccatgcccga ttgaaaaatg
      481 acccgattgc aacgcatcgc ccaataacac aatatttcca tcataggcct tttcatgact
      541 taacgtcatg aaattacgcc atttcaatcc tgattgagca ataacaggct ttccgttcaa
      601 atcagattca aatacggatg caataaacgc ttctgcttgg tctgcagaca tttcgtctag
      661 acctgacttt agatacgtct cttcgctaca ttcaacaaca aaagtactca tgtcactcga
      721 atatttatag gcatgagcga caaaaacacc tttagaatgc gctttaaaaa tcaggttcat
      781 ttcattaaaa agattaggcg tgccatacca catgtagcga ttcgtgccat attcaatgtt
      841 cggttttagc gcttcac
//
