LOCUS       sequence-118245-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, 
            Wang Z, Koffas MA
  TITLE     Improvement of catechin production in Escherichia coli through 
            combinatorial metabolic engineering.
  JOURNAL   Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
  PUBMED    25527438
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2014-12-17"; volume: "28C:43-53. doi"; pages: " 
            10.1016/j.ymben.2014.12.002"
COMMENT     Sequence Label: p168
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             234..256
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    271..295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(295..314)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(296..314)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(348..395)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     391..409
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(448..492)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 attctttatc gttgatcacg ccataaatca ctttggcacc tttatcctga aaggttttga
       61 taataactgc tttgctcggg ctaaccggac ccggacgaac cagcagaaag gtcggataac
      121 caaaacccag gcttgctttg gtaataaact gaccaataaa accggtgcca ccaacaacca
      181 gggtacgatt tttggtcatg ctcggaattg caccgctaac ggtcatatgt atatctcctt
      241 cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat
      301 agtgagtcgt attaatttcg cgggatcgag atcgatctcg atcctagcaa aaaacccctc
      361 aagacccgtt tagaggcccc aaggggttat gctagttatt gctcagcggt ggcagcagtt
      421 taggttaatt aagctgcgac tagtagacga gtccatgtgc tggcgttcaa atttcgcagc
      481 agcggtttct ttaccagact cgagttaacg acgacgtttc ggaggcagtg caggaggagg
      541 acctgcacga cgtttttcgg tttcttcttc ttgcggcagc ggcagcagac ctttttcacg
      601 acatgcatca acggcacctg taaacatatc ttccaggctg tgtttaaatt cgaagcccat
      661 ctctttcagt tttttgctgc taaagtgaat gttggtcagg ttttcttcga tgcctttgaa
      721 ttttttcgga acgttgtatt tcgggtattt ctcgttcagc agttttgcaa tatcgtgaat
      781 ggttgcatca tggctgctac aaatataacg accctcggct tttgcatgtt catacag
//
