LOCUS       sequence-118284-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is an envelope gene encoding plasmid to make retrogradely 
            lentiviral vector. If you use this plasmid, you can make type E 
            envelope coating virus particle with NeuRet..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Kato S, Kobayashi K, Kobayashi K
  TITLE     Improved transduction efficiency of a lentiviral vector for 
            neuron-specific retrograde gene transfer by optimizing the junction 
            of fusion envelope glycoprotein.
  JOURNAL   J Neurosci Methods. 2014 Apr 30;227:151-8. doi: 
            10.1016/j.jneumeth.2014.02.015. Epub 2014 Mar 5.
  PUBMED    24613797
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2014-04-30"; volume: "227:151-8. doi"; pages: " 
            10.1016/j.jneumeth.2014.02.015. Epub 2014 Mar 5"
COMMENT     Sequence Label: pCAGGS-FuG-E
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     311..330
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    329..384
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     430..449
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(623..655)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 gggatctcca taagagaaga gggacagcta tgactgggag tagtcaggag aggaggaaaa
       61 atctggctag taaaacatgt aaggaaaatt ttagggatgt taaagaaaaa aataacacaa
      121 aacaaaatat aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga
      181 cagcaggggg ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag
      241 ccagcatatg ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat
      301 gatttgccct cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg
      361 caatgaaaat aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt
      421 ccccataatt tttggcagag ggaaaaagat ctgctagcta tagttctaga ggtaccggtt
      481 gttgggggct gcaggaattc cgtttttttt tttttttttc ataaaaatta aaaactcaaa
      541 tataattgag gcctctttga gcatggtatc acaagttgat ttggtccaaa catgaagaat
      601 ctgttgtgca ggatttgagt tactttccaa gtcggttcat ctctatgtct gtataaatct
      661 gtcttttctt ggtgtgcttt aatttaatgc aaagatggat accaactcgg agaaccaaga
      721 atagtccaat gattaaccct atgat
//
