LOCUS       sequence-118291-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, 
            mVenus-tagged RhoC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Zawistowski JS, Sabouri-Ghomi M, Danuser G, Hahn KM, Hodgson L
  TITLE     A RhoC biosensor reveals differences in the activation kinetics of 
            RhoA and RhoC in migrating cells.
  JOURNAL   PLoS One. 2013 Nov 5;8(11):e79877. doi: 
            10.1371/journal.pone.0079877. eCollection 2013.
  PUBMED    24224016
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-11-5"; volume: "8(11):e79877. doi"; pages: " 
            10.1371/journal.pone.0079877. eCollection 2013"
COMMENT     Sequence Label: RhoC FLARE.sc mCer, mVenus - wt
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     205..227
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     289..308
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     350..369
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        350..368
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    369..393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        409..518
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 cgtcgtttag tgaccgtcag atcactagat gctttattgc ggtagtttat cacagttaaa
       61 ttgctaacgc cagtctcgaa cttaacgtgc agaagttggt cgtgaggcac tgggcaggta
      121 agtatcgggc cctttgtgcg gggggagcgg ctcgnggctg tccgcggggg gacggctgcc
      181 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
      241 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
      301 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc
      361 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc
      421 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
      481 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatggcgcg
      541 aggccttctg gaagaacagt attttgaatt gacgcaagaa agcaagaaag ctgcttcaag
      601 aaatagacaa gagattacag ataaagatca cactgttagt cggcttgaag aagcaaacag
      661 catgctaacc aaagatattg aaatattaag aagagagaat gaagagctaa cagagaaaat
      721 gaagaa
//
