LOCUS       sequence-118292-         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, 
            mVenus-tagged RhoC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Zawistowski JS, Sabouri-Ghomi M, Danuser G, Hahn KM, Hodgson L
  TITLE     A RhoC biosensor reveals differences in the activation kinetics of 
            RhoA and RhoC in migrating cells.
  JOURNAL   PLoS One. 2013 Nov 5;8(11):e79877. doi: 
            10.1371/journal.pone.0079877. eCollection 2013.
  PUBMED    24224016
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-11-5"; volume: "8(11):e79877. doi"; pages: " 
            10.1371/journal.pone.0079877. eCollection 2013"
COMMENT     Sequence Label: RhoC FLARE.sc mCer, mVenus - wt
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     98..119
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(396..647)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNXEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(438..459)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggcagcttgc cggtggtgca gatcaacttc agggtcagct tgccgtaggt ggcatcgccc
       61 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
      121 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccattgcggc cgcngatcca
      181 cctttagtag aaccttcacc agaaccaggt ttaccagaac cagaagtaga tccaccttta
      241 gtagaacctt caccagaacc aggtttacca gaaccagaag tagatccacc tttagtagaa
      301 ccttcaccag aaccaggttt accagaacca gaagtagatc cacctttagt agaaccttca
      361 ccagaaccag gtttaccaga accagaagtt aagcttttgt acagctcgtc catgccgaga
      421 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
      481 tctttgctca gcttggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
      541 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcnatg
      601 ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc ggt
//
