LOCUS       sequence-118298-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gltA targeting guide RNA E. coli , Low Phosphate Induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  TITLE     SUPERSEDE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCASCADE-gltA2
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(92..194)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(224..850)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     755..774
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      859..930
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
       61 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
      121 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
      181 ttacgtgccg atcaacgtct cattttcgcc agatatcgac gtcttaagac ccactttcac
      241 atttaagttg tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct
      301 ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag
      361 tagtaggtgt ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac
      421 ctaaagtaaa atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt
      481 gttggcataa aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg
      541 tacctaaatg tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag
      601 cgttattacg taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt
      661 gcctatctaa catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc
      721 aatacaatgt aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt
      781 cgattccgac ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc
      841 tagacatcat ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 ggg
//
