LOCUS       sequence-118341-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GPR171-Tango
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             786..794
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgagc tcggatccac
      121 tagtccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgcg ccaccatgaa
      181 gacgatcatc gccctgagct acatcttctg cctggtattc gccgactaca aggacgatga
      241 tgacgccagc atcgatatga caaactcttc ttttttctgt cctgtctaca aggatctgga
      301 gcccttcacc tacttttttt acctcgtctt tctggtgggt atcatcggta gctgctttgc
      361 cacttgggct ttcatccaga agaacacgaa tcaccgctgc gtgtcaatct atttgataaa
      421 ccttctgacc gcggactttc ttctgacgct ggcccttccc gtcaaaattg tcgtagactt
      481 gggcgttgcc ccgtggaagc tgaagatctt tcattgccaa gtgacagcct gcctgatata
      541 cattaacatg tacctgagca tcatattcct cgcttttgtt tccattgacc gctgcctcca
      601 actgacacat tcttgtaaaa tctatagaat tcaggagcca ggattcgcca agatgatttc
      661 tacagtcgtg tggctgatgg tgcttctgat tatggtccct aacatgatga ttccaattaa
      721 ggatatcaag gaaaagtcta acgttggctg catggagttt aaaaaggaat ttggtcgcaa
      781 ttggcacctc cttaccaact tcatatgtgt tgccatcttt ctgaatttct ccgctatcat
      841 cctcatcagc aattgtctgg tgatccgcca gctctacaga aataaggaca acgaaaatta
      901 tccaaatgtg aagaaggcgc tgataaatat actgttggtg accactggat atatcatttg
      961 ttttgtgccc tatcac
//
