LOCUS       sequence-118352-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 490 / 553, Emission: 507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..52
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             56..76
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             80..100
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             113..802
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 tgaaccgtca gatccgctag ccaccatgga tccaaaaaag aagagaaagg tagatccaaa
       61 aaagaagaga aaggtagatc caaaaaagaa gagaaaggta ccggtcgcca ccatgaacac
      121 cccgggaatt aacctgatca aggaggacat gcgcgtgaag gtgcacatgg agggcaacgt
      181 gaacggccac gccttcgtga tcgagggcga gggcaagggc aagccctacg agggcaccca
      241 gaccgccaac ctgaccgtga aggagggcgc ccccctgccc ttcagctacg acatcctgac
      301 caccgccgtg cactacggca accgggtgtt caccaagtac cccgaggaca tccccgacta
      361 cttcaagcag agcttccccg agggctacag ctgggagcgc accatgacct tcgaggacaa
      421 gggcatctgc accatccgca gcgacatcag cctggagggc gactgcttct tccagaacgt
      481 gcgcttcaag ggcaccaact tcccccccaa cggccccgtg atgcagaaga agaccctgaa
      541 gtgggagccc agcaccgaga agctgcacgt gcgcgacggc ctgctggtgg gcaacatcaa
      601 catggccctg ctgctggagg gcggcggcca ctacctgtgc gacttcaaga ccacctacaa
      661 ggccaagaag gtggtgcagc tgcccgacgc ccacttcgtg gaccaccgca tcgagatcct
      721 gggcaacgac agcgactaca acaaggtgaa gctgtacgag cacgccgtgg cccgctacag
      781 ccccctgccc agccaggtgt ggtccggaga agggcaaggg caaggg
//
