LOCUS       sequence-118498-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-BQ_6 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-BQ_6
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(190..280)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(308..335)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(427..513)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(529..546)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
       61 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      121 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      181 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      241 gagcggatac atatttgaat gtatttagaa aaataaacaa cggaccgaaa gagtttgtag
      301 aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc tggcagttta
      361 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca aatccgctcc
      421 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
      481 ccagtctttc gactgagcct ttcgttttat ttgaagcttt cattattaat gatggtggtg
      541 atgatgtccc gagccagcct caccttggcc agctcctgcc tctccctgtc cagcaccagc
      601 ttcaccttgt cccgcccccg cagaacctgt accagcacct cccaattgtt gctgaccagc
      661 agatcctgtt cccgcacctc cgagctgctg ctgacctgcc tcaccctgcc ctgctcccgc
      721 ttctccttgt ccagcccctg cttcgccctg tcctgcaccc gccgaccccg tccctgcacc
      781 tcctagctgt tgctgcccgg cagacccagt cccagcaccc cct
//
