LOCUS       sequence-118538-        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express an additional copy of Bem1, integrated at TRP locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Jost AP, Weiner OD
  TITLE     Probing Yeast Polarity with Acute, Reversible, Optogenetic 
            Inhibition of Protein Function.
  JOURNAL   ACS Synth Biol. 2015 Jun 2.
  PUBMED    26035630
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Jun 2."
COMMENT     Sequence Label: pCyc-Bem1
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taatacgact cactataggg ggccagcgtg gccgtttaaa catttaaatg gccggccttc
       61 tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga gtagtatgtt
      121 gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact cttggtattc
      181 tcgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat tgaccagagc
      241 caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg gagtgcctga
      301 actattttta tatgctttta caagacttga aattttcctt gcaataaccg ggtcaattgt
      361 tctctttcta ttgggcacac atataatacc cagtaagtca gcatcggaac cacacaatta
      421 taagcaaagg gggtacaatc attacaccac aacacaacac ccagtttaat ttccatgagt
      481 tcaaatcaag agtggtccca agatatatta aaacaattga atctacgaga ctctcgggaa
      541 aaaaaccatg agattgatgc tagatatttc aacgccttcc agcaattgtc tcaaaaattg
      601 aatcaattat ccacaacaac aaatactctt ctacaggacc aatcaagcaa ttcagaaact
      661 ttgacaaatc taataaactc taaagaaatt gaaaacttga aacttgaaaa caccgagttg
      721 ataactaact taaataattt aattatcaat aatgaaaaac taacacaaac aatcaatgcc
      781 agagatcaaa ccataaaatc attaaccaaa atcaacgaca aattgaataa taaaattgat
      841 gcattaaatt tggaaataaa cgagaaaaac aaaactatag aattgattaa tgatgaagta
      901 ttgacaaatc aaatacaatt gaatgtattg agaagtaaac tagagttgtt ggagaggtaa
      961 acccagaaag cattcatcgc gttaattaat agacaactca ttttatagag tatatacaac
     1021 tgtataagat agtaataaaa atatcgcact cacgtaaaca cttaatcata atattaattc
     1081 gtatataatt tagctatttg ctta
//
