LOCUS       sequence-118576-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express GFP-tagged dominat negative let60, in which Lysine 16 is 
            mutated to asparagine, in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Iwasa H, Kuroyanagi H, Maimaiti S, Ikeda M, Nakagawa K, Hata Y
  TITLE     Characterization of RSF-1, the Caenorhabditis elegans homolog of the
            Ras-association domain family protein 1.
  JOURNAL   Exp Cell Res. 2013 Feb 1;319(3):1-11. doi: 
            10.1016/j.yexcr.2012.10.008. Epub 2012 Oct 26.
  PUBMED    23103556
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.yexcr.2012.10.008"; journalName: "Exp Cell Res"; date: 
            "2013-02-1- 1"; volume: "319"; issue: "3"; pages: "1-11"
COMMENT     Sequence Label: pClneoEGFPC-let60 K16N
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(633..654)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 attatttgac acttcttctt cttttgtggc ttattattgt cgtgacgctc acgatgcttg
       61 cgaatttctc taacaagtgt gtaaaatgct tcatcaactc ccatacgcgt tttggcagat
      121 gtgtcgacat tcggaatacc gtaacccttt gctgtctcac tgactgttcg gaagtcgact
      181 gatcgtgatg acaaatcaca tttattccct accaagacca taggaacatc atctgaatcc
      241 tttaccctcc gaatctgctc gcggtagtta gcgacattct cgaaagattt agcctcgttg
      301 acggcgaaaa ccaacagaaa tccttcgcct gtcctcatgt actgatcacg catcgccgaa
      361 tattcttctt gtccggcggt atccaatatg tcgaggaggc atgtctcacc gtctatcaca
      421 acctgctttc tgtagctgtc ctctatggtc gggtcgtatt cttcgacaaa gtgattctgg
      481 atgagttgaa tggtgagtgc tgaattacca actcctccat ctccaactac cacaagcttg
      541 tactccgtca tgaattcgaa gcttgagctc gagatctgag tccggccgga cttgtacagc
      601 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
      661 cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc
      721 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
      781 ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg
      841 tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg c
//
