LOCUS       sequence-118633-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal fusion to oxBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha 
            VV, Snapp EL
  TITLE     A palette of fluorescent proteins optimized for diverse cellular 
            environments.
  JOURNAL   Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670.
  PUBMED    26158227
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-9"; volume: "6:7670. doi"; pages: " 
            10.1038/ncomms8670"
COMMENT     Sequence Label: oxBFP
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFISTTGKLPVPWPTLVTTLSHGVQVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg aggggcgagg gcgagggcga tgccaccaac
      121 ggcaagctga ccctgaagtt catcagtact accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgagcca cggcgtgcag gtgttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaccta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcgtcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaacttcaa cagccacaac atctatatca tggccgtcaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccgccac aacgtggagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacagccac
      601 tacctgagca cccagtccgt gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tccgcaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
//
