LOCUS       sequence-118638-         591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducbile color change and cancer regulation: FlpO recombinase 
            dependent expression of KrasG12D cMyc and SV40 large T antigen with 
            doxycycline inducible downregulation through tetKRAB system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Callesen MM, Arnadottir SS, Lyskjaer I, Orntoft MW, Hoyer S, 
            Dagnaes-Hansen F, Liu Y, Li R, Callesen H, Rasmussen MH, Berthelsen 
            MF, Thomsen MK, Schweiger PJ, Jensen KB, Laurberg S, Orntoft TF, 
            Elverlov-Jakobsen JE, Andersen CL
  TITLE     A genetically inducible porcine model of intestinal cancer.
  JOURNAL   Mol Oncol. 2017 Nov;11(11):1616-1629. doi: 10.1002/1878-0261.12136. 
            Epub 2017 Oct 10.
  PUBMED    28881081
REFERENCE   2  (bases 1 to 591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1878-0261.12136"; journalName: "Mol Oncol"; date: 
            "2017-11"; volume: "11"; issue: "11"; pages: "1616-1629"
COMMENT     Sequence Label: 
            LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-S
            V40LT-IRES-KRABoff-PA-TRE-loxP257-RIR
FEATURES             Location/Qualifiers
     source          1..591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(31..48)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    37..261
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctagatcata atcagcccgg gggtgatcag cctcgactgt gccttctagt tgccagccat
       61 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
      121 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
      181 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
      241 gggatgcggt gggctctatg gaaccagctg gttgtttatt gcagcttata atggttacaa
      301 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
      361 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcttgttta ttgcagctta
      421 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      481 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcttgtt
      541 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca c
//
