LOCUS       sequence-118653-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of iCre recombinase from the broadly active CAG
            promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pAYC
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..36
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttcgccacca tggtgcccaa gaagaagagg aaagtctcca acctgctgac tgtgcaccaa
       61 aacctgcctg ccctccctgt ggatgccacc tctgatgaag tcaggaagaa cctgatggac
      121 atgttcaggg acaggcaggc cttctctgaa cacacctgga agatgctcct gtctgtgtgc
      181 agatcctggg ctgcctggtg caagctgaac aacaggaaat ggttccctgc tgaacctgag
      241 gatgtgaggg actacctcct gtacctgcaa gccagaggcc tggctgtgaa gaccatccaa
      301 cagcacctgg gccagctcaa catgctgcac aggagatctg gcctgcctcg cccttctgac
      361 tccaatgctg tgtccctggt gatgaggaga atcagaaagg agaatgtgga tgctggggag
      421 agagccaagc aggccctggc ctttgaacgc actgactttg accaagtcag atccctgatg
      481 gagaactctg acagatgcca ggacatcagg aacctggcct tcctgggcat tgcctacaac
      541 accctgctgc gcattgccga aattgccaga atcagagtga aggacatctc ccgcaccgat
      601 ggtgggagaa tgctgatcca cattggcagg accaagaccc tggtgtccac agctggtgtg
      661 gagaaggccc tgtccctggg ggttaccaag ctggtggaga gatggatctc tgtgtctggt
      721 gtggctgatg accccaacaa ctacctgttc tgccgggtca gaaagaatgg tgtggctgcc
      781 ccttctgcca cctcccaact gtccacccgg gccctggaag ggatctttga ggccacccac
      841 cgcctgatct atggtgccaa ggatgactct gg
//
